Human parechovirus infections in Canada
Human parechovirus infections in Canada
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DOI：
10.3201/eid1206.051675
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发表时间：
2006-06-01
影响因子：
11.8
通讯作者：
Boivin, Guy
中科院分区：
文献类型：
--
作者：
Abed, Yacine;Boivin, Guy
建立了一种新的逆转录-聚合酶链反应检测方法，用于鉴定1985年至2004年回收的28株加拿大人双埃柯病毒（HPeV）分离株，包括20株HPeV-1，3株HPeV-2和5株HPeV-3。除1株外，所有HPeV-1分离株均与Harris参考株在遗传上不同。1株HPeV-2分离株与威廉姆森株相关;另外2株与康涅狄格株相关。HPeV-3分离株聚在一起。75%的分离株是在典型的肠道病毒季节回收的。除1例患者外，所有患者均为儿童，HPeV-1、HPeV-2和HPeV-3患者的平均年龄分别为14.6个月、6.3个月和0.7个月。所有HPeV-2和HPeV-3感染的儿童均因诊断为病毒血症或脓毒症而住院。HPeV-1感染的儿童中有50%的病例诊断为细支气管炎，少数病例为肺炎和肠炎。两名感染患者（1名白血病儿童和1名78岁女性）分别死于感染性休克和严重肺炎。
A new reverse transcription-polymerase chain reaction assay was developed for identification of 28 Canadian human parechovirus (HPeV) isolates, including 20 HPeV-1, 3 HPeV-2, and 5 HPeV-3, recovered from 1985 to 2004. All HPeV-1 isolates but 1 were genetically distinct from the Harris reference strain. One HPeV-2 isolate was related to the Williamson strain; the other 2 were related to the Connecticut strain. HPeV-3 isolates clustered together. Seventy-five percent of isolates were recovered during the typical enterovirus season. All patients but 1 were children with a mean age of 14.6 months, 6.3 months, and 0.7 months for HPeV-1, HPeV-2, and HPeV-3 patients, respectively. All HPeV-2- and HPeV-3-infected children were hospitalized with a diagnosis of viremia or sepsis. HPeV-1-infected children had bronchiolitis diagnosed in 50% of the cases, with few cases of pneumonia and enteritis. Two infected patients (1 child with leukemia and a 78-year-old woman) died of septic shock and severe pneumonia, respectively.