A novel route for synthesis of nanocrystalline hydroxyapatite from eggshell waste
A novel route for synthesis of nanocrystalline hydroxyapatite from eggshell waste
复制标题
DOI：
10.1007/s10856-007-3069-7
复制
发表时间：
2007-09-01
影响因子：
3.7
通讯作者：
Kumar, T. S. Sampath
中科院分区：
文献类型：
--
作者：
Krishna, D. Siva Rama;Siddharthan, A.;Kumar, T. S. Sampath
利用微波技术将蛋壳废弃物加工成纳米晶羟基磷灰石（HA）。为了突出蛋壳作为钙前体在HA（OHA）合成中的优势，还使用合成氢氧化钙按照类似的程序形成HA（SHA），并与市售的纯HA（CHA）进行比较。采用X射线粉末衍射（XRD）、傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和比表面积测定等方法对所合成的羟基磷灰石进行了表征。通过XRD图谱中的特征宽峰、TEM显微照片中的长33 - 50 nm和宽8 - 14 nm的片晶和由比表面积测量计算的尺寸，揭示了OHA的纳米晶体性质。FT-IR光谱显示HA的特征谱带和碳酸根离子的峰。晶胞参数计算表明形成了B型碳酸化HA。OHA在硬度和密度方面比SHA和CHA表现出上级的可烧结性，这可能是由于其球晶结构的表面积更大。体外溶出度研究表明，OHA在磷酸盐缓冲液中具有更长的稳定性，使用成骨细胞的细胞培养试验确定了OHA的生物相容性。
The eggshell waste has been value engineered to a nanocrystalline hydroxyapatite ( HA) by microwave processing. To highlight the advantages of eggshell as calcium precursor in the synthesis of HA (OHA), synthetic calcium hydroxide was also used to form HA (SHA) following similar procedure and were compared with a commercially available pure HA (CHA). All the HAs were characterized by X-ray powder diffraction (XRD) method, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and specific surface area measurements. Nanocrystalline nature of OHA is revealed through characteristic broad peaks in XRD patterns, platelets of length 33-50 nm and width 8-14 nm in TEM micrograph and size calculations from specific surface area measurements. FT-IR spectra showed characteristic bands of HA and additionally peaks of carbonate ions. The cell parameter calculations suggest the formation of carbonated HA of B-type. The OHA exhibits superior sinterability in terms of hardness and density than both SHA and CHA may be due to larger surface area of its spherulite structure. The in vitro dissolution study shows longer stability in phosphate buffer and cell culture test using osteoblast cells establishes biocompatibility of OHA.