The potential roles of long non‐coding RNAs in lipopolysaccharide‐induced human peripheral blood mononuclear cells as determined by microarray analysis
The potential roles of long non‐coding RNAs in lipopolysaccharide‐induced human peripheral blood mononuclear cells as determined by microarray analysis
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DOI：
10.1002/2211-5463.12556
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发表时间：
2018-12
期刊：
影响因子：
2.6
通讯作者：
Anqiang Zhang;Hong-xiang Lu;D. Wen;Jian-hui Sun;J. Du;Xu Wang;W. Gu;Jianxin Jiang
中科院分区：
文献类型：
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作者：
Anqiang Zhang;Hong-xiang Lu;D. Wen;Jian-hui Sun;J. Du;Xu Wang;W. Gu;Jianxin Jiang
脓毒症是由宿主对感染的异常和过度反应引起的。长非编码 RNA (lncRNA) 参与多种细胞功能，包括炎症和免疫。然而，迄今为止，还没有系统地尝试鉴定在先天免疫反应诱导后表达发生变化的lncRNA。在这项研究中，我们使用微阵列平台分析了经脂多糖 (LPS) 处理的外周血单核细胞 (PBMC) 中的整体 lncRNA 和 mRNA 表达变化。在筛选的 40 914 个 lncRNA 中，响应 LPS，596 个显着上调，250 个显着下调（校正后 P < 0.05）。在筛选的 34 236 个 mRNA 中，802 个上调，549 个下调。功能注释分析表明，lncRNA相关的差异表达mRNA主要富集于宿主免疫和炎症反应中。这项研究首次提供了 LPS 介导的人类 PBMC 变化的 lncRNA 和 mRNA 转录组图谱。这些发现可能为了解脓毒症免疫病理学中涉及的 lncRNA 提供潜在的见解。
Sepsis arises from an aberrant and excessive host response to infection. Long noncoding RNAs (lncRNAs) are involved in multiple cellular functions, including inflammation and immunity. However, to date there has been no systematic attempt to identify lncRNAs whose expression is changed after the induction of the innate immune response. In this study, we profiled global lncRNA and mRNA expression changes in peripheral blood mononuclear cells (PBMCs) treated with lipopolysaccharide (LPS) using a microarray platform. Of the 40 914 lncRNAs screened, 596 were significantly upregulated and 250 were significantly downregulated (corrected P < 0.05) in response to LPS. Of the 34 236 mRNAs screened, 802 were upregulated and 549 were downregulated. Functional annotation analysis indicated that lncRNA‐associated differentially expressed mRNAs were primarily enriched in host immune and inflammatory responses. This study provides the first lncRNA and mRNA transcriptomic landscape of LPS‐mediated changes in human PBMCs. These findings may provide potential insights into lncRNAs involved in the immunopathology of sepsis.