Structure and thermotropic properties of phosphatidylethanolamine and its N-methyl derivatives.
Structure and thermotropic properties of phosphatidylethanolamine and its N-methyl derivatives.
复制标题
磷脂酰乙醇胺及其N-甲基衍生物的结构和热致性质。
DOI：
10.1021/bi00306a030
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Shipley,GG
中科院分区：
文献类型：
--
作者：
Mulukutla,S;Shipley,GG
Satish Mulukutla和G. GrahamShipley* 摘要：用差示扫描量热法和X射线衍射法研究了1，2-二肉豆蔻酰-异-甘油基-3-磷酸乙醇胺（DMPE）及其N，N-二甲基（DMPE）和N-单甲基（mmDMPE）衍生物的水化双层膜的结构和热致性。对于DMPE、mmDMPE和dmDMPE，多分子层分散体（~ 50重量%水）在49. 2、42.3和30.7 ℃，1，2-二肉豆蔻酰-n-甘油-3-磷酸胆碱的相应值出现在23 ℃。因此，双层链熔融转变随着N-甲基化的增加而降低，如最初针对相应的棕榈酰基系列所报道的[Vaughan，D. J.，& Keough，K. M. 04 The Dog of the Woman（1974）47，158-161]。这种转变是可逆的冷却，和DMPE，mmDMPE，和dmDMPE形成原来的双层凝胶相与旋转无序的碳氢化合物链包装在一个六方lattice. After在-4 ℃下长时间孵育，双层凝胶相被证明是亚稳态的，和转换到低温“结晶”相发生与碳氢化合物链采用特定的包装模式。对于DMPE、mmDMPE和dmDMPE，当“晶体”双层相转化为双层凝胶相时，发生单吸热转变或双吸热转变。对于棕榈酰系列观察到类似的行为模式。具有六方堆积的亚稳双层凝胶相的相对缓慢的动力学转化
Satish Mulukutla and G. GrahamShipley* abstract: The structure and thermotropic properties of hy-drated bilayers of l, 2-dimyristoyl-i «-glycero-3-phospho-ethanolamine (DMPE) and its A-monomethyl (mmDMPE) and/V, iV-dimethyl (dmDMPE) derivatives have been inves-tigated by differential scanning calorimetry and X-ray dif-fraction. For DMPE, mmDMPE, and dmDMPE, multila-mellar dispersions (~ 50 wt% water) show chainmelting bilayer gel-* bilayer liquid-crystal transitions (onset) at49. 2, 42.3, and 30.7 C, respectively, with the corresponding value for 1, 2-dimyristoyl-j «-glycero-3-phosphocholine occurring at 23 C. Thus, the bilayer chain melting transition decreases with increasing N-methylation, as originally reported for the corresponding palmitoyl series [Vaughan, D. J., & Keough, K. M.(1974) FEBS Lett. 47, 158-161]. This transition is reversible on cooling, and DMPE, mmDMPE, and dmDMPE form the original bilayer gel phase with the rotationally dis-ordered hydrocarbon chains packed in a hexagonal lattice.Following prolonged incubation at-4 C, the bilayer gel phase is shown to be metastable, and conversion to a low-temperature “crystalline” phase occurs with the hydrocarbon chains adopting a specific packing mode. For DMPE, mmDMPE, and dmDMPE, either a single or a double endothermic transition occurs as the “crystal” bilayer phase converts to the bilayer gel phase. A similar pattern of behavior is observed for the palmitoyl series. The relatively slow kinetic conversion of the metastable bilayer gel phase with hexagonally packed