1H and 13C NMR assignments for five anthraquinones from the mangrove endophytic fungus Halorosellinia sp (No. 1403)
1H and 13C NMR assignments for five anthraquinones from the mangrove endophytic fungus Halorosellinia sp (No. 1403)
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DOI：
10.1002/mrc.2078
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发表时间：
2007-11-01
影响因子：
2
通讯作者：
Lin, Yong-Cheng
中科院分区：
文献类型：
--
作者：
Xia, Xue-Kui;Huang, Hua-Rong;Lin, Yong-Cheng
本文报道了两个新天然产物1，4，5，6，7，9-六羟基-2-甲氧基-7-甲基-5β，9β，8aβ，6α，10aα-六氢并-10(10ah)-酮(1)和1，4，6-trihydroxy-2-methoxy-7-methylanthracene-9，10-二酮(2)的核磁共振氢谱归属。这些化合物都是从采自南中国海区的1403号海洋内生真菌中分离得到的。化合物3和4为首次从海洋真菌中分离得到。通过一维和二维核磁共振波谱方法，包括COSY、HMQC、HMBC和NOE，以及HREIMS，确定了它们的结构。在我们的细胞毒性实验中，化合物5对KB和KBV-200细胞具有细胞毒性，其IC50分别为1.40和2.58mU g/ml。此外，还讨论了化合物1、2、3和4的可能的生物起源关系。版权所有(C)2007 John Wiley&Sons，Ltd.
We report the unambiguous assignments of the H-1 and C-13 NMR spectra of two new natural products, namely, 1,4,5,6,7,9-hexahydroxy-2-methoxy-7-methyl-5 beta,9 beta,8a beta, 6 alpha,10a alpha-hexahydroanthracen-10 (10aH)-one (1) and 1,4,6-trihydroxy-2-methoxy-7-methylanthracene-9, 10-dione (2), together with three known anthraquinones. These compounds were all isolated from the marine endophytic fungus No. 1403 collected from the South China Sea. Compounds 3 and 4 were isolated from the marine fungus for the first time. The structures were elucidated by the spectroscopic methods 1D and 2D NMR including COSY, HMQC, HMBC and NOE, and HREIMS. In our cytotoxicity assays, compound 5 showed cytotoxicity toward KB and KBv-200 cells with IC50 of 1.40 and 2.58 mu g/ml, respectively. In addition, the plausible biogenic relationship of compounds 1, 2, 3 and 4 is discussed. Copyright (c) 2007 John Wiley & Sons, Ltd.