Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer
Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer
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DOI：
10.1016/j.intimp.2022.108735
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发表时间：
2022-04-08
影响因子：
5.6
通讯作者：
He, Yulong
中科院分区：
文献类型：
--
作者：
Liu, Huifang;Wu, Jing;He, Yulong
目的：具有免疫球蛋白和ITIM结构域的T细胞免疫受体（TIGIT）是一种新型的免疫抑制分子。本研究旨在探讨胃癌（GC）中B细胞TIGIT的表达及TIGIT（+）CD20（+）B细胞的功能。方法：收集194例胃癌患者的肿瘤组织石蜡包埋切片及临床病理资料。采用双重免疫组化方法检测B细胞TIGIT的表达。采用多重免疫荧光初步探讨TIGIT(+)CD20(+) B细胞与CD8(+) T细胞耗竭的关系。结果：在GC中，瘤内、瘤周和三级淋巴结构(TLS)中均观察到TIGIT(+)CD20(+) B细胞。瘤周 TIGIT(+)CD20(+) B 细胞浸润较高的 GC 患者临床结果较差，可从辅助化疗 (ACT) 中获益。在GC组织中，PD-1(+)CD8(+) T细胞比TIGIT(+)CD20(+) B细胞更接近TIGIT(+)CD20(+) B细胞。 结论：瘤周TIGIT(+)CD20(+) B细胞浸润是GC患者的独立预后预测因子，也是ACT选择的潜在生物标志物。 TIGIT(+)CD20(+)B细胞可能影响GC中CD8(+)T细胞的耗竭。
Objective: T cell immunoreceptor with immunoglobulin and ITIM domains (TIGIT) is a novel immunosuppressive molecule. This study aimed to investigate the expression of TIGIT on B cells and the function of TIGIT(+)CD20(+) B cells in gastric cancer (GC).Methods: Tumor tissue paraffin-embedded sections and clinicopathological data from 194 patients with GC were collected. Dual immunohistochemistry was performed to detect the expression of TIGIT on B cells. Multiplex immunofluorescence was used to initially explore the relationship between TIGIT(+)CD20(+) B cells and the exhaustion of CD8(+) T cells.Results: In GC, TIGIT(+)CD20(+) B cells were observed in intratumor, peritumor, and tertiary lymphoid structures (TLS). Patients with GC having high peritumoral TIGIT(+)CD20(+) B cells infiltration had inferior clinical outcomes and could benefit from adjuvant chemotherapy (ACT). In GC tissues, PD-1(+)CD8(+) T cells were more closer to TIGIT(+)CD20(+) B cells than to TIGIT(+)CD20(+) B cells.Conclusions: Peritumoral TIGIT(+)CD20(+)& nbsp;B cells infiltration was an independent prognostic predictor for patients with GC and a potential biomarker for ACT selection. TIGIT(+)CD20(+)& nbsp;B cells might affect the exhaustion of CD8(+) T cells in GC.