Changes in apoplast protein pattern suggest an early role of cell wall structure remodelling in flagellin-triggered basal immunity
Changes in apoplast protein pattern suggest an early role of cell wall structure remodelling in flagellin-triggered basal immunity
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DOI：
10.1007/s10535-011-0226-0
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发表时间：
2012-04
影响因子：
1.5
通讯作者：
E. Szabó;Á. Szatmári;É. Hunyadi-Gulyás;E. Besenyei;L. R. Zsíros;Z. Bozsó;P. Ott
中科院分区：
文献类型：
--
作者：
E. Szabó;Á. Szatmári;É. Hunyadi-Gulyás;E. Besenyei;L. R. Zsíros;Z. Bozsó;P. Ott
植物叶片质外体是病原菌侵染后与植物接触的动态区室。最早的相互作用事件之一是受体介导的细菌表面分子的感知，如鞭毛蛋白或其他保守的微生物相关分子模式（MAMP）。质外体蛋白可能在基础抗性（BR）或模式触发免疫（PTI）中发挥作用。在这里，蛋白质组学的方法进行了水溶性-潜在的最移动的-质外体蛋白从鞭毛蛋白处理的烟草（烟草）叶片。由于BR/PTI的快速性似乎对其效力至关重要，因此早在接种后2.5和7 h取样。蛋白质通过双向聚丙烯酰胺凝胶电泳（2D-PAGE）分离，并通过液相色谱串联质谱（LC-MS/MS）鉴定。与水接种对照相比，来自28个蛋白质点的49种不同蛋白质的密度发生了变化。在EBR诱导过程中，出现了11个蛋白质点。其中有糖水解酶和氧化还原活性蛋白，除了病程相关的蛋白质，预测植物细胞壁结构的修改和更直接的抗菌效应的最早的变化有关BR/PTI。
The leaf apoplast is a dynamic compartment in contact with plant pathogenic bacteria after infection. Among the very first interaction events is the receptor-mediated perception of bacterial surface molecules such as flagellin or other conserved microbe-associated molecular patterns (MAMPs). Apoplast proteins likely play a role in basal resistance (BR) or pattern-triggered immunity (PTI). Here, a proteomic approach was carried out on water soluble — potentially the most mobile — apoplast proteins from flagellin-treated tobacco (Nicotiana tabacum) leaves. As the quickness of BR/PTI seems crucial for its efficacy, samples were taken as early as 2.5 and 7 h post inoculation. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and identified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Forty-nine different proteins from 28 protein spots changed in their density compared to the water-inoculated control. Eleven protein spots appearedde novoin response to EBR induction. There are glycohydrolases and redox-active proteins besides pathogenesis-related proteins among them, predicting plant cell wall structural modifications and more direct antimicrobial effectors as earliest changes related to BR/PTI.