Expression of genes involved in sumoylation in the Drosophila germline
Expression of genes involved in sumoylation in the Drosophila germline
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DOI：
10.1016/j.gep.2008.08.001
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发表时间：
2009-01-01
影响因子：
1.2
通讯作者：
Kobayashi, Satoru
中科院分区：
文献类型：
--
作者：
Hashiyama, Kazuya;Shigenobu, Shuji;Kobayashi, Satoru
据报道，通过共价添加小泛素相关修饰物（SUMO）蛋白对蛋白质的翻译后修饰可以调节广泛的细胞过程。然而，在发育过程中编码sumoylation机制的基因的时空表达模式在很大程度上是未知的。在这里，我们报道了5个sumo化基因Uba2、Aos1、smt3、Ulp1和lesswright （lwr）在果蝇种系中的表达。这五个基因的转录本通过全载原位杂交在整个早期胚胎中检测到，而它们主要在晚期胚胎的种系祖细胞或极细胞中表达。这些基因在卵发生和精子发生过程中也在种系中表达。最后，我们发现SUMO蛋白在极细胞和配子体细胞的细胞核中富集。鉴于大部分SUMO底物是核蛋白，这些数据表明SUMO化在种系中高度活跃。我们的数据为理解sumoylation如何调节种系发育提供了基础。(C) 2008 Elsevier B.V.版权所有
Post-translational modification of proteins by the covalent addition of small ubiquitin-related modifier (SUMO) proteins has been reported to regulate a wide range of cellular processes. However, the spatio-temporal expression pattern of genes encoding the sumoylation machinery during development is largely unknown. Here, we report the expression of five sumoylation genes, Uba2, Aos1, smt3, Ulp1, and lesswright (lwr), in the Drosophila germline. Transcripts from all five genes were detected throughout the early embryo by whole-mount in situ hybridization, while they were predominantly expressed in the germline progenitors, or pole cells, in late stage embryos. These genes were also expressed in the germline during oogenesis and spermatogenesis. Finally, we found that SUMO protein was enriched in the nuclei of pole cells and gametogenic cells. Given that a large fraction of SUMO substrates are nuclear proteins, these data suggest that sumoylation is highly active in the germline. Our data provide a basis for understanding how sumoylation regulates germline development. (C) 2008 Elsevier B.V. All rights reserved.