ISOLATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME VIRUS (ISOLATE ATCC VR-2332) IN NORTH-AMERICA AND EXPERIMENTAL REPRODUCTION OF THE DISEASE IN GNOTOBIOTIC PIGS
ISOLATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME VIRUS (ISOLATE ATCC VR-2332) IN NORTH-AMERICA AND EXPERIMENTAL REPRODUCTION OF THE DISEASE IN GNOTOBIOTIC PIGS
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DOI：
10.1177/104063879200400201
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发表时间：
1992-04-01
影响因子：
1.5
通讯作者：
CHLADEK, D
中科院分区：
文献类型：
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作者：
COLLINS, JE;BENFIELD, DA;CHLADEK, D
对明尼苏达州一猪场最近发生的猪不孕症和呼吸综合征（SIRS）兽疫进行了调查。对猪群中猪、新生仔猪和死产胎儿的检查显示，仔猪出现间质性肺炎、单核淋巴细胞脑炎和单核淋巴细胞心肌炎，母猪出现局灶性脑血管炎。胎儿没有显微镜下的病变。未确定不孕和呼吸综合征的原因。因此，人们试图通过实验再现这种疾病。11只3日龄的非生物制剂仔猪鼻内暴露于动物流行病仔猪的组织浆液中，在暴露后2-4天出现食欲不振和发热，并出现与现场暴发相似的间质性肺炎和脑炎。在非生仔猪中盲传2次后，组织匀浆在连续细胞系CL2621上培养，分离到细胞病变病毒（ATCC VR-2332），暂命名为SIRS病毒。鼻内暴露于SIRS病毒的新生仔猪出现的临床症状和显微镜下病变与暴露于组织匀浆的仔猪相同，并且从其肺部重新分离出病毒。这是美国第一例SIRS病毒分离株，符合Koch在非生仔猪中产生呼吸道形式疾病的假设，也是第一例在连续细胞系（CL2621）上分离和繁殖该病毒的报道。该病毒被指定为美国型培养集VR-2332。
A recent epizootic of swine infertility and respiratory syndrome (SIRS) in a Minnesota swine herd was investigated. Examination of a sow, neonatal piglets, and stillborn fetuses obtained during the epizootic from the affected herd revealed interstitial pneumonitis, lymphomononuclear encephalitis, and lymphomononuclear myocarditis in the piglets and focal vasculitis in the brain of the sow. Fetuses did not have microscopic lesions. No cause for the infertility and respiratory syndrome was determined. Therefore, attempts were made to experimentally reproduce the disease. Eleven 3-day-old gnotobiotic piglets exposed intranasally to tissue homogenates of piglets from the epizootic became inappetent and febrile by 2-4 days postexposure and had interstitial pneumonitis and encephalitis similar to that seen in the field outbreak. After 2 blind passages in gnotobiotic piglets, tissue homogenates were cultured on continuous cell line CL2621, and a cytopathic virus (ATCC VR-2332), provisionally named SIRS virus, was isolated. Gnotobiotic piglets exposed intranasally to the SIRS virus developed clinical signs and microscopic lesions that were the same as those in piglets exposed to the tissue homogenates, and the virus was reisolated from their lungs. This is the first isolate of SIRS virus in the United States that fulfills Koch's postulates in producing the respiratory form of the disease in gnotobiotic piglets and the first report of isolation and propagation of the virus on a continuous cell line (CL2621). The virus is designated as American Type Culture Collection VR-2332.