Outbreak of Zika Virus Infection, Chiapas State, Mexico, 2015, and First Confirmed Transmission by Aedes aegypti Mosquitoes in the Americas
Outbreak of Zika Virus Infection, Chiapas State, Mexico, 2015, and First Confirmed Transmission by Aedes aegypti Mosquitoes in the Americas
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DOI：
10.1093/infdis/jiw302
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发表时间：
2016-11-01
影响因子：
6.4
通讯作者：
Weaver, Scott C.
中科院分区：
文献类型：
--
作者：
Guerbois, Mathilde;Fernandez-Salas, Ildefonso;Weaver, Scott C.
背景经过几十年的默默无闻，寨卡病毒（ZIKV）自2015年以来在美洲传播，并伴有先天性小头畸形和格林-巴利综合征。虽然这些流行病可能涉及埃及伊蚊的传播，但没有报道媒介参与的直接证据，这促使人们猜测可能涉及其他蚊子，如致倦库蚊。分析疑似ZIKV感染患者的血清中的病毒RNA和抗体。在患者家中和周围收集蚊子并测试ZIKV.Results.aEuro积分在119名疑似ZIKV感染的患者中，25名（21%）通过在体征和症状（包括皮疹、关节痛、头痛、瘙痒、肌痛和发烧）发作后1-8天收集的血清的RT-PCR确认。在采集的796只蚊虫中，A.通过RT-PCR，埃及伊蚊在55个池中的15个（27.3%）中产生ZIKV检测。在C.致倦肌来自血清和蚊子的ZIKV序列显示出单系关系，提示从危地马拉引入的点源。结论：aEuro积分这些结果证明ZIKV持续、快速向北进展进入北美，具有典型的轻度疾病表现，并涉及A.埃及伊蚊首次成为北美的主要病媒。
Background. After decades of obscurity, Zika virus (ZIKV) has spread through the Americas since 2015 accompanied by congenital microcephaly and Guillain-Barre syndrome. Although these epidemics presumably involve transmission by Aedes aegypti, no direct evidence of vector involvement has been reported, prompting speculation that other mosquitoes such as Culex quinquefasciatus could be involved.Methods.aEuro integral We detected an outbreak of ZIKV infection in southern Mexico in late 2015. Sera from suspected ZIKV-infected patients were analyzed for viral RNA and antibodies. Mosquitoes were collected in and around patient homes and tested for ZIKV.Results.aEuro integral Of 119 suspected ZIKV-infected patients, 25 (21%) were confirmed by RT-PCR of serum collected 1-8 days after the onset of signs and symptoms including rash, arthralgia, headache, pruritus, myalgia, and fever. Of 796 mosquitoes collected, A. aegypti yielded ZIKV detection by RT-PCR in 15 of 55 pools (27.3%). No ZIKV was detected in C. quinquefasciatus. ZIKV sequences derived from sera and mosquitoes showed a monophyletic relationship suggestive of a point source introduction from Guatemala.Conclusions.aEuro integral These results demonstrate the continued, rapid northward progression of ZIKV into North America with typically mild disease manifestations, and implicate A. aegypti for the first time as a principal vector in North America.