Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment
Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment
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DOI：
10.1271/bbb.120155
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发表时间：
2012-07-01
影响因子：
1.6
通讯作者：
Uchida, Takafumi
中科院分区：
文献类型：
--
作者：
Fukuda, Tomokazu;Katayama, Masafumi;Uchida, Takafumi
猪是一种重要的农用和医用动物。然而，与小鼠和人类来源的细胞系相比，猪来源的细胞系的数量相对有限。本研究建立了猴空泡病毒40大T片段(SV40T)的逆转录病毒条件表达系统，为高效建立猪胚胎成纤维细胞系奠定了基础。建立的细胞系显示出高水平的细胞增殖和对细胞衰老的抵抗力。染色体分析显示，84%的细胞具有正常核型。Cre重组酶的瞬时表达使我们能够从基因组中剔除SV40T片段。这一研究工具的开发将使我们能够快速建立来自各种动物的新细胞系。
The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.