Supramolecular organization of photosynthetic complexes in membranes of Roseiflexus castenholzii
Supramolecular organization of photosynthetic complexes in membranes of Roseiflexus castenholzii
复制标题
DOI：
10.1007/s11120-015-0179-9
复制
发表时间：
2016-01-01
影响因子：
3.7
通讯作者：
Blankenship, Robert E.
中科院分区：
文献类型：
--
作者：
Majumder, Erica L. -W.;Olsen, John D.;Blankenship, Robert E.
人们利用电子显微镜、原子力显微镜和生物化学研究了丝状缺氧光养生物Roseiflexuscastenholzii的光合膜。光捕获反应中心复合物的电子显微镜产生了一个 3D 模型，该模型与来自 Thermochromatium tepidum 的 RC-LH1 的已解析晶体结构对齐，其中 H 亚基已被移除。整个膜的原子力显微镜产生了光合电子传递链中主要蛋白质的超分子组织的图像。结果表明，膜是松散堆积的，没有附属天线蛋白或更高阶的结构。
The photosynthetic membranes of the filamentous anoxygenic phototroph Roseiflexus castenholzii have been studied with electron microscopy, atomic force microscopy, and biochemistry. Electron microscopy of the light-harvesting reaction center complex produced a 3D model that aligns with the solved crystal structure of the RC-LH1 from Thermochromatium tepidum with the H subunit removed. Atomic force microscopy of the whole membranes yielded a picture of the supramolecular organization of the major proteins in the photosynthetic electron transport chain. The results point to a loosely packed membrane without accessory antenna proteins or higher order structure.