Protein kinases in divergent eukaryotes: identification of protein kinase activities regulated during trypanosome development.
Protein kinases in divergent eukaryotes: identification of protein kinase activities regulated during trypanosome development.
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不同真核生物中的蛋白激酶：锥虫发育过程中调节的蛋白激酶活性的鉴定。
DOI：
10.1073/pnas.90.7.2656
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发表时间：
1993
影响因子：
11.1
通讯作者：
Carter,V
中科院分区：
文献类型：
--
作者：
Parsons,M;Valentine,M;Carter,V
在真核生物谱系的早期分化的生物体中，蛋白激酶的作用相对来说还没有被探索。在这项研究中，我们确定原始寄生原生动物具有多种蛋白-丝氨酸激酶，并通过敏感的免疫印迹技术推测蛋白-酪氨酸激酶的存在。为了进一步探索蛋白激酶在寄生虫发育中的作用，我们检测了原生动物布氏锥虫生活史中八种可复性蛋白激酶的活性。六种蛋白-丝氨酸/苏氨酸激酶的活性在发育过程中受到调节，有几种不同的调节模式。此外，在分裂的细胞中检测到一个89 kDa的蛋白激酶，但在未分裂的细胞中没有检测到。我们的数据表明，即使是最原始的真核生物也拥有大量的蛋白激酶，包括蛋白酪氨酸激酶和蛋白质丝氨酸/苏氨酸激酶。这些数据进一步表明，蛋白激酶可能在原生动物的增殖和分化调节中发挥关键作用。
The role of protein kinases in organisms that diverged early in the eukaryotic lineage is relatively unexplored. In this study, we determined that primitive parasitic protozoa possess multiple protein-serine kinases and inferred the presence of protein-tyrosine kinases through sensitive immunoblotting techniques. To further explore the role of protein kinases in parasite development, we examined the activity of eight renaturable protein kinases during the life cycle of the protozoan parasite Trypanosoma brucei. The activities of six protein-serine/threonine kinases were regulated during development, with several distinct patterns of regulation. In addition, an 89-kDa protein kinase was detected in dividing cells but not in nondividing cells. Our data indicate that even the most primitive eukaryotes possess a large complement of protein kinases, including protein-tyrosine kinases as well as protein-serine/threonine kinases. The data further suggest that protein kinases may play a pivotal role in regulation of proliferation and differentiation in protozoa.