The zinc-binding protein chordc1 undergoes complex diurnal changes in mRNA expression during mouse brain development
The zinc-binding protein chordc1 undergoes complex diurnal changes in mRNA expression during mouse brain development
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DOI：
10.1007/s11064-006-9271-z
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发表时间：
2007-02-01
影响因子：
4.4
通讯作者：
Landry, Charles F.
中科院分区：
文献类型：
--
作者：
Gerstner, Jason R.;Landry, Charles F.
最近在小鼠下丘脑中发现了Chordc1mRNA的昼夜变化。这份报告显示，Chordc1mRNA在整个成人大脑中有节奏地变化，最高水平的表达出现在暗-光转换前后。Chordc1的节律循环模式在不同的明-暗时间表下保持不变，成人全脑的分析显示出与幼年动物不同的昼夜模式(出生后(P)6)。对成年海马、前额叶皮质和小脑的分析证实了这些观察结果，通过原位杂交在成年动物和P6动物之间的比较表明，Chordc1在发育过程中经历了协调但不同的mRNA丰度日变化。此外，从胚胎第17天开始的Chordc1表达的发育概况显示Chordc1的区域分布与其成年模式一致。这些结果表明，Chordc1mRNA在发育过程中受到复杂而广泛的转录调控，并参与哺乳动物大脑的昼夜节律和/或稳态机制。
Diurnal changes in Chordc1 mRNA were recently described in mouse hypothalamus. This report shows that Chordc1 mRNA changes rhythmically throughout the entire adult brain with highest expression levels occurring around the dark-light transition. The rhythmic cycling pattern of Chordc1 was retained under various light-dark schedules and analysis of adult whole brain revealed diurnal patterns that were different than young animals (postnatal day (P) 6). Analysis of adult hippocampus, prefrontal cortex and cerebellum confirmed these observations and a comparison between adult and P6 animals using in situ hybridization indicated that Chordc1 underwent coordinated but altered diurnal changes in mRNA abundance during development. Further, a developmental profile of Chordc1 expression beginning at embryonic day 17 revealed a regional distribution of Chordc1 consistent with its adult pattern. These results suggest that Chordc1 mRNA is under complex and widespread transcriptional regulation during development and implicate Chordc1 in circadian and/or homeostatic mechanisms in mammalian brain.