Low dose of tetrodotoxin reduces neuropathic pain behaviors in an animal model
Low dose of tetrodotoxin reduces neuropathic pain behaviors in an animal model
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DOI：
10.1016/s0006-8993(00)02451-3
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发表时间：
2000-07-14
期刊：
影响因子：
2.9
通讯作者：
Chung, JM
中科院分区：
文献类型：
--
作者：
Lyu, YS;Park, SK;Chung, JM
我们推测，河豚毒素（TTX）敏感性钠通道在受损背根神经节（DRG）神经元的积累起着至关重要的作用，在产生异位放电和机械异常性疼痛周围神经损伤后。使用神经病理性疼痛的节段脊神经（L5）结扎模型，通过检查TTX对受影响的后爪的机械敏感性的影响来测试该假设。使用长期植入的聚乙烯管将不同浓度的TTX局部应用于L5 DRG。数据显示，以远低于阻断动作电位传导所需剂量的低剂量（12.5-50 nM）应用TTX，可显著升高爪中的机械阈值，以进行足退缩，这是异常性疼痛行为减少的迹象。这些数据表明，TTX敏感的钠通道亚型在维持神经病理性疼痛动物模型的异常性疼痛行为中起重要作用。(C)2000 Elsevier Science B. V.保留所有权利。
We hypothesize that the accumulation of tetrodotoxin (TTX) sensitive sodium channels in injured dorsal root ganglion (DRG) neurons plays a critically important role in the generation of ectopic discharges and mechanical allodynia after peripheral nerve injury. Using the segmental spinal nerve (L5) ligation model of neuropathic pain, this hypothesis was tested by examining the effect of TTX on the mechanical sensitivity of the affected hind paw. Various concentrations of TTX were applied topically to the L5 DRG by using chronically implanted polyethylene tubing. The data showed that application of TTX at low doses (12.5-50 nM), which are far less than those needed for blocking action potential conduction, produced a significant elevation of mechanical threshold in the paw for foot withdrawals, a sign of reduced allodynic behaviors. The data suggest that TTX-sensitive subtypes of sodium channels play an important role in maintaining allodynic behaviors in an animal model of neuropathic pain. (C) 2000 Elsevier Science B.V. All rights reserved.