STUDIES ON RABBIT LYMPHOCYTES IN VITRO .2. INDUCTION OF BLAST TRANSFORMATION WITH ANTISERA TO SIX IGG ALLOTYPES AND SUMMATION WITH MIXTURES OF ANTISERA TO DIFFERENT ALLOTYPES
STUDIES ON RABBIT LYMPHOCYTES IN VITRO .2. INDUCTION OF BLAST TRANSFORMATION WITH ANTISERA TO SIX IGG ALLOTYPES AND SUMMATION WITH MIXTURES OF ANTISERA TO DIFFERENT ALLOTYPES
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DOI：
10.1084/jem.122.4.813
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发表时间：
1965-01-01
影响因子：
15.3
通讯作者：
SELL, S
中科院分区：
文献类型：
--
作者：
GELL, PGH;SELL, S
当将针对兔IgG的所有6种充分表征的同种异型决定簇（As1、2、3、4、5和6）的特异性抗血清添加到从具有适当IgG同种异型的供体兔获得的淋巴细胞培养物中时，其能够诱导胚细胞转化和DNA合成。针对2种不同同种异型决定簇的抗血清的混合物诱导转化和DNA合成的“总和”超过针对相同同种异型决定簇的2种抗血清的混合物的效果。无论混合的抗血清是针对由相同基因座或不同基因座控制的同种异型决定簇，都观察到这种总和效应。针对双纯合子兔的两种同种异型决定簇的2种抗血清混合物发生总和的发现（As1，6）表明，来自外周血的淋巴细胞可能被引发仅产生IgG的2条多肽链中的一条或另一条，而不是两条。
Specific antisera directed against all 6 of the well characterised allotypic determinants of rabbit IgG (Asl, 2,3,4,5, and 6) are capable of inducing blast transformation and DNA synthesis when added to lymphocyte cultures obtained from donor rabbits having the appropriate IgG allotype. Mixtures of antisera directed against 2 different allotypic determinants induce a "summation" of transformation and DNA synthesis over and above the effect of mixtures of 2 antisera directed against the same allotypic determinant. This summation effect is observed regardless of whether the antisera which have been mixed are directed against allotypic determinants controlled by the same locus or by different loci. The finding that summation occurs with mixtures of 2 antisera directed against both the allotypic determinants of a double homozygote rabbit (Asl, 6) suggests that lymphocytes from the peripheral blood may be primed to produce only one or the other of the 2 polypeptide chains of IgG, but not both.