Bioaugmentation treatment of mature landfill leachate by new isolated ammonia nitrogen and humic acid resistant microorganism.
Bioaugmentation treatment of mature landfill leachate by new isolated ammonia nitrogen and humic acid resistant microorganism.
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DOI：
10.4014/jmb.1401.01066
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发表时间：
2014-07
影响因子：
2.8
通讯作者：
Dahai Yu;Jiyu Yang;F. Teng;Li-Lin Feng;Xuexun Fang;Hejun Ren
中科院分区：
文献类型：
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作者：
Dahai Yu;Jiyu Yang;F. Teng;Li-Lin Feng;Xuexun Fang;Hejun Ren
成熟垃圾渗滤液具有高浓度氨氮（NH3-N）和腐殖酸（HA）的特点，由于有机物组分毒性大、可生物降解性差，给生物处理技术带来了挑战。本研究采用生物强化技术对垃圾渗滤液进行降解，通过驯化获得一株对氨氮和腐殖酸具有抗性的芽孢杆菌（Bacillus cereus，简称B）。蜡状肠球菌（Enterococcus casseliflavus）（缩写为E. casseliflavus Jlu）。筛选出的菌株对NH3-N和HA具有较强的耐受能力，能显著提高垃圾渗滤液的COD、NH3-N和HA去除率，提高生物强化降解效率。驯化菌仅需3d即可去除70.0%左右的COD，而未驯化（本地）菌需9d才能达到类似的去除率。采用正交试验进一步提高COD和NH3-N的去除率。在最佳工艺条件下，E. casseliflavus Jlu和B.蜡状芽孢杆菌Jlu在降解2d后分别提高到86.0%和90.0%。首次发现该菌株对渗滤液中氨氮和腐殖酸的去除率分别达到50%和40%以上。
The mature landfill leachate, which is characterized by a high concentration of ammonia nitrogen (NH3-N) and humic acid (HA), poses a challenge to biotreatment methods, due to the constituent toxicity and low biodegradable fraction of the organics. In this study, we applied bioaugmentation technology in landfill leachate degradation by introducing a domesticated NH3-N and HA resistant bacteria strain, which was identified as Bacillus cereus (abbreviated as B. cereus Jlu) and Enterococcus casseliflavus (abbreviated as E. casseliflavus Jlu), respectively. The isolated strains exhibited excellent tolerant ability for NH3-N and HA and they could also greatly improved the COD (chemical oxygen demand), NH3-N and HA removal rate, and efficiency of bioaugmentation degradation of landfill leachate. Only 3 days was required for the domesticated bacteria to remove about 70.0% COD, compared with 9 days' degradation for the undomesticated (autochthonous) bacteria to obtain a similar removal rate. An orthogonal array was then used to further improve the COD and NH3-N removal rate. Under the optimum condition, the COD removal rate in leachate by using E. casseliflavus Jlu and B. cereus Jlu increased to 86.0% and 90.0%, respectively after, 2 days of degradation. The simultaneous removal of NH3-N and HA with more than 50% and 40% removal rate in leachate by employing the sole screened strain was first observed.