Endomorphin-1 mediates 2 Hz but not 100 Hz electroacupuncture analgesia in the rat
Endomorphin-1 mediates 2 Hz but not 100 Hz electroacupuncture analgesia in the rat
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DOI：
10.1016/s0304-3940(99)00670-9
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发表时间：
1999-10-22
影响因子：
2.5
通讯作者：
Han, JS
中科院分区：
文献类型：
--
作者：
Han, Z;Jiang, YH;Han, JS
本研究旨在阐明内源性内吗啡肽 -1（EM1）在低频或高频电针镇痛中可能的作用。以辐射热甩尾潜伏期（TFL）作为痛觉的指标，大鼠鞘内（i.t.）注射10 μl抗EM1血清（EM1 - AS）或正常兔血清（NRS，作为对照），然后进行2Hz或100Hz电针刺激30分钟。鞘内注射1∶10和1∶100稀释的EM1 - AS可减弱2Hz电针诱导的镇痛作用，但1∶1000稀释的则无此作用。当鞘内注射任何稀释度的EM1 - AS时，未观察到对100Hz电针镇痛有这种抑制作用。这些结果表明，在脊髓水平，EM1参与2Hz电针镇痛，但不参与100Hz电针镇痛。（C）1999年，爱尔兰爱思唯尔科学有限公司。版权所有。
This work was designed to elucidate the possible involvement of endogenous endomorphin-1 (EM1) in analgesia induced by electroacupuncture of low or high frequencies. Taking radiant heat tail flick latency (TFL) as an indication of nociception, rats were subjected to intrathecal (i.t.) injection of 10 mu l antiserum against EM1 (EM1-AS) or normal rabbit serum (NRS, as control) and then followed by 2 or 100 Hz electroacupuncture stimulation for 30 min. The analgesia induced by 2 Hz electroacupuncture was attenuated by i.t. injection of EM1-AS at 1:10 and 1:100 but not at 1:1000 dilution. No such suppressive effect was observed for 100 Hz EA analgesia when EM1-AS was injected i.t. at any dilutions. These results indicate that EM1 is involved in 2 Hz but not 100 Hz electroacupuncture analgesia at spinal level. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.