Hydration changes accompanying the binding of minor groove ligands with DNA
Hydration changes accompanying the binding of minor groove ligands with DNA
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DOI：
10.1529/biophysj.106.097451
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发表时间：
2007-02-01
影响因子：
3.4
通讯作者：
Petty, Jeffrey T.
中科院分区：
文献类型：
--
作者：
Degtyareva, Natalya N.;Wallace, Bret D.;Petty, Jeffrey T.
4 '，6-二脒基-2-苯基吲哚（DAPI）、神经肽和喷他脒是具有末端-CdNH 2）（2）（+）基团的小沟结合剂。利用渗透压技术和荧光光谱研究了它们与（AATT）（2）序列小沟结合时的水合变化。DAPI对结合位点的亲和力随着溶液的渗透压摩尔浓度的增加而降低，导致在结合时获得35 +/- 1沃茨。采用竞争荧光法，以DNA-DAPI复合物为荧光报告分子，测定了其他两种配体的结合常数和水合变化。在它们与（AATT）（2）结合位点结合后，netropsin和喷他脒分别获得26 +/- 3和34 +/- 2额外的水合沃茨。的水合作用的变化进行了讨论的背景下的配体和构象变化的DNA中的末端官能团。
4',6-Diamidino-2-phenylindole (DAPI), netropsin, and pentamidine are minor groove binders that have terminal -CdNH2)(2)(+) groups. The hydration changes that accompany their binding to the minor groove of the (AATT)(2) sequence have been studied using the osmotic stress technique with fluorescence spectroscopy. The affinity of DAPI for the binding site decreases with the increasing osmolality of the solution, resulting in acquisition of 35 +/- 1 waters upon binding. A competition fluorescence assay was utilized to measure the binding constants and hydration changes of the other two ligands, using the DNA-DAPI complex as the fluorescence reporter. Upon their association to the (AATT)(2) binding site, netropsin and pentamidine acquire 26 +/- 3 and 34 +/- 2 additional waters of hydration, respectively. The hydration changes are discussed in the context of the terminal functional groups of the ligands and conformational changes in the DNA.