Cystic fibrosis sputum supports growth and cues key aspects of Pseudomonas aeruginosa physiology
Cystic fibrosis sputum supports growth and cues key aspects of Pseudomonas aeruginosa physiology
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DOI：
10.1128/jb.187.15.5267-5277.2005
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发表时间：
2005-08-01
影响因子：
3.2
通讯作者：
Whiteley, M
中科院分区：
文献类型：
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作者：
Palmer, KL;Mashburn, LM;Whiteley, M
人类机会致病菌铜绿假单胞菌引起囊性纤维化（CF）患者持续气道感染。为了建立这些慢性感染，铜绿假单胞菌必须在CF患者肺部的高粘性痰中生长和增殖。在这项研究中，我们使用Affyssin基因芯片微阵列来研究使用CF痰作为碳和能量的唯一来源生长的铜绿假单胞菌的生理学。我们的研究结果表明，CF痰容易支持高密度铜绿假单胞菌的生长。此外，CF痰液中存在多种信号，其降低游泳运动性并过早激活铜绿假单胞菌中的假单胞菌喹诺酮信号细胞间信号级联。在CF痰液中也诱导了对常见CF肺居民金黄色葡萄球菌的裂解至关重要的铜绿假单胞菌因子，并且在CF痰液中的多微生物生长期间增加了铜绿假单胞菌的竞争力。
The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum.