A model of emotional stress-induced binge eating in female mice with no history of food restriction
A model of emotional stress-induced binge eating in female mice with no history of food restriction
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DOI：
10.1111/gbb.12613
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发表时间：
2019-10-23
影响因子：
2.5
通讯作者：
Brown, Robyn M.
中科院分区：
文献类型：
--
作者：
Anversa, Roberta G.;Campbell, Erin J.;Brown, Robyn M.
暴饮暴食是导致肥胖和相关健康并发症的主要因素。特别是对于女性来说，压力等负面情绪会强烈影响饮食行为和暴食事件。在啮齿类动物中模拟这种类型的暴食提出了挑战：首先，在啮齿类动物中通常观察到压力诱导的厌食症，因此需要温和的压力源以观察促食欲作用。其次，许多研究报告使用卡路里限制来观察所需的行为;但这并不一定反映人类的状况。因此，本研究的目的是建立一个不依赖于热量限制的情绪压力诱导的暴食模型。将雌性和雄性C57 BL/6 J小鼠分为自由采食组（n = 20/性别）和食物限制组（n = 20/性别），将其进一步分为对照组和暴露于挫折应激的组（n = 10/组）。在2个循环中，向所有小鼠提供间歇性的高适口性食物。在每个周期结束时，压力组经历15分钟的挫折事件，其中非常可口的食物在笼子里，但无法接近。然后，两组人都可以自由进入15分钟。与对照组相比，随意进食的沮丧雌性小鼠表现出暴食样行为（P = .0001）。值得注意的是，这种行为在雄性中不存在。卵巢切除术对暴食行为没有影响。总的来说，这些数据验证了一种新的模型，情绪压力引起的暴食特定于雌性小鼠，不需要热量限制，不受卵巢激素的驱动。
Overeating is a major contributing factor to obesity and related health complications. For women, in particular, negative emotions such as stress strongly influence eating behavior and bingeing episodes. Modeling this type of binge eating in rodents presents challenges: firstly, stress-induced anorexia is commonly observed in rodents therefore a mild stressor is required in order to observe an orexigenic effect. Second, many studies report using calorie restriction to observe the required behavior; yet this does not necessarily reflect the human condition. Thus, the aim of this study was to develop a model of emotional stress-induced bingeing independent of caloric restriction. Female and male C57BL/6J mice were divided into ad libitum (n = 20 per sex) and food-restricted (n = 20 per sex) groups which were both further split into a control group and a group exposed to frustration stress (n = 10 per group). All mice were provided intermittent access to a highly palatable food in 2 cycles. At the end of each cycle the stress group was subjected to a 15-minute frustration episode where highly palatable food was within the home cage but inaccessible. Both groups were then given free access for 15 minutes. Frustrated female mice from the ad libitum displayed binge-like behavior compared with controls (P = .0001). Notably, this behavior was absent in males. Ovariectomy had no impact on binge-like behavior. Collectively, these data validate a novel model of emotional stress-induced binge eating specific to female mice which does not require caloric restriction and is not driven by ovarian hormones.