Structure of the Ribosomal RNA Decoding Site Containing a Selenium-Modified Responsive Fluorescent Ribonucleoside Probe.
Structure of the Ribosomal RNA Decoding Site Containing a Selenium-Modified Responsive Fluorescent Ribonucleoside Probe.
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DOI：
10.1002/anie.201611700
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发表时间：
2017-03-01
期刊：
影响因子：
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通讯作者：
Srivatsan SG
中科院分区：
文献类型：
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作者：
Nuthanakanti A;Boerneke MA;Hermann T;Srivatsan SG
全面了解RNA在真实的时间和原子水平上的结构-功能关系将对我们进一步理解RNA在生物学中的功能产生深远的影响。在这里，我们描述了多功能核苷探针的第一个例子，包含构象敏感的荧光团和异常的X射线衍射标记（5-硒代尿嘧啶），这使得在平衡和3D下的RNA构象和识别的相关性。探针掺入细菌核糖体RNA解码位点，荧光报告抗生素结合，并提供衍射信息，以确定结构，而不扭曲天然RNA折叠。此外，通过比较溶液结合数据和晶体结构，我们了解了探针如何检测配体诱导的RNA构象变化。总的来说，我们的核苷探针代表了一类新的生物物理工具，将补充功能RNA研究的可用工具。
Comprehensive understanding of the structure–function relationship of RNA both in real time and at atomic level will have a profound impact in advancing our understanding of RNA functions in biology. Here, we describe the first example of a multifunctional nucleoside probe, containing a conformation‐sensitive fluorophore and an anomalous X‐ray diffraction label (5‐selenophene uracil), which enables the correlation of RNA conformation and recognition under equilibrium and in 3D. The probe incorporated into the bacterial ribosomal RNA decoding site, fluorescently reports antibiotic binding and provides diffraction information in determining the structure without distorting native RNA fold. Further, by comparing solution binding data and crystal structure, we gained insight on how the probe senses ligand‐induced conformational change in RNA. Taken together, our nucleoside probe represents a new class of biophysical tool that would complement available tools for functional RNA investigations.