The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and L-DOPA
The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and L-DOPA
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DOI：
10.1016/j.soilbio.2009.02.029
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发表时间：
2009-06-01
影响因子：
9.7
通讯作者：
DeForest, Jared L.
中科院分区：
文献类型：
--
作者：
DeForest, Jared L.
本实验的目的是评估是否土壤储存和处理方法显着影响测量潜在的原位酶活性在酸性森林土壤。更具体地说，目标是确定。(1)土壤储存时间和温度、缓冲液中土壤泥浆的持续时间和模型基质的年龄显著影响了六种常用的土壤胞外酶的活性。在2小时内收集和分析土壤酶活性被认为是潜在的原位酶活性的最佳量度和所有统计比较的基准。相同土壤的子样品储存在4摄氏度或-20摄氏度。除了温度控制外，土壤还经历了两次实验处理。首先，在收集后2、7、14和21天分析酶活性。第二，立即添加MUB连接的底物（即，
The purpose of this experiment was to evaluate whether soil storage and processing methods significantly influence measurements of potential in situ enzyme activity in acidic forest soils. More specifically, the objectives were to determine if. (1) duration and temperature of soil storage; (2) duration of soil slurry in buffer; and (3) age of model substrates significantly influence the activity of six commonly measured soil extracellular enzymes using methylumbelliferone (MUB)-linked substrates and L-dihydroxyphenylalanine (L-DOPA). Soil collected and analyzed for enzyme activity within 2 h was considered the best measure of potential in situ enzyme activity and the benchmark for all statistical comparisons. Sub-samples of the same soil were stored at either 4 degrees C or -20 degrees C. In addition to the temperature manipulation, soils experienced two more experimental treatments. First, enzyme activity was analyzed 2, 7,14, and 21 days after collection. Second, MUB-linked substrate was added immediately (i.e.