In vivo murine left ventricular pressure-volume relations by miniaturized conductance micromanometry
In vivo murine left ventricular pressure-volume relations by miniaturized conductance micromanometry
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DOI：
10.1152/ajpheart.1998.274.4.h1416
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发表时间：
1998-04-01
影响因子：
4.8
通讯作者：
Kass, DA
中科院分区：
文献类型：
--
作者：
Georgakopoulos, D;Mitzner, WA;Kass, DA
小鼠是创建人类疾病基因工程模型的首选物种。为了研究小鼠在体左心室（LV）收缩和舒张的详细力学，我们开发了一个小型化的电导压力计系统。将双电极压力-容积导管通过LV尖推进到主动脉根部，对α-氯醛糖-甘露醇麻醉的动物进行器械化。定制电子器件提供与腔体体积相关的时变电导。基线血流动力学与清醒动物的数值相似：心率、收缩末期和舒张末期压力以及心室压力相对于时间的最大一阶导数（dP/dt（max））分别为634 +/- 14次/min、112 +/- 4 mmHg、5.3 +/- 0.8 mmHg和11，777 +/- 732 mmHg/s。下腔静脉阻塞减少前负荷时导管每搏输出量与超声主动脉血流探头相关（r（2）= 0.98）。该操作产生79 +/-21 mmHg/μ l的收缩末期弹性，82 +/-5.6 mmHg的预负荷-可恢复的搏出功，以及699 +/-100 mmHg·s（-1）·μ l（-1）的dP/dt（max）-舒张末期容积关系的斜率，并且这些关系随着急性变力性干预而可预测地变化。对照标准化时变弹性曲线与人体数据相似，进一步支持了物种之间的可比腔室力学。这种新的方法应该大大有助于评估血液灌注小鼠心脏的心血管功能。
The mouse is the species of choice for creating genetically engineered models of human disease. To study detailed systolic and diastolic left ventricular (LV) chamber mechanics in mice in vivo, we developed a miniaturized conductance-manometer system. alpha-Chloralose-urethananesthetized animals were instrumented with a two-electrode pressure-volume catheter advanced via the LV apex to the aortic root. Custom electronics provided time-varying conductances related to cavity volume. Baseline hemodynamics were similar to values in conscious animals: 634 +/- 14 beats/min, 112 +/- 4 mmHg, 5.3 +/- 0.8 mmHg, and 11,777 +/- 732 mmHg/s for heart rate, end-systolic and end-diastolic pressures, and maximum first derivative of ventricular pressure with respect to time (dP/dt(max)), respectively. Catheter stroke volume during preload reduction by inferior vena caval occlusion correlated with that by ultrasound aortic flow probe (r(2) = 0.98). This maneuver yielded end-systolic elastances of 79 +/- 21 mmHg/mu l, preload-recruitable stroke work of 82 +/- 5.6 mmHg, and slope of dP/dt(max)-end-diastolic volume relation of 699 +/- 100 mmHg.s(-1).mu l(-1), and these relations varied predictably with acute inotropic interventions. The control normalized time-varying elastance curve was similar to human data, further supporting comparable chamber mechanics between species. This novel approach should greatly help assess cardiovascular function in the blood-perfused murine heart.