Protocol for inducing cellular ablation in the mouse atrioventricular conduction system.
Protocol for inducing cellular ablation in the mouse atrioventricular conduction system.
复制标题
DOI：
10.1016/j.xpro.2023.102145
复制
发表时间：
2023-03-17
期刊：
影响因子：
--
通讯作者：
Munshi, Nikhil V.
中科院分区：
文献类型：
--
作者：
Wang, Lin;Dela Rosa, Jared Gabriel L.;Munshi, Nikhil V.
房室传导系统（AVCS）是心房和心室腔之间的主要电连接，其损伤可导致各种心脏传导障碍。在这里，我们提供了一个协议，选择性损害的小鼠AVCS，以研究其在损伤过程中的反应。我们描述了他莫昔芬诱导的细胞消融，通过心电图检测房室传导阻滞，并定量组织学和免疫荧光标记物来分析AVCS。该方案可用于研究与AVCS损伤修复和再生相关的机制。有关本方案使用和执行的完整详细信息，请参见Wang et al.（2021年）。小鼠房室传导系统的可重复消融损伤模型新生和成年小鼠中他莫昔芬诱导的不同途径新生和成年小鼠心电图的简化说明AVCS组织免疫组织化学技术的详细演练出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。房室传导系统（AVCS）是心房和心室腔之间的主要电连接，其损伤可导致各种心脏传导障碍。在这里，我们提供了一个协议，选择性损害的小鼠AVCS，以研究其在损伤过程中的反应。我们描述了他莫昔芬诱导的细胞消融，通过心电图检测房室传导阻滞，并定量组织学和免疫荧光标记物来分析AVCS。该方案可用于研究与AVCS损伤修复和再生相关的机制。
Damage to the atrioventricular conduction system (AVCS), the main electrical connection between the atrial and ventricular chambers, can result in a variety of cardiac conduction disorders. Here, we provide a protocol for selective damage of the mouse AVCS to study its response during injury. We describe tamoxifen-induced cellular ablation, detection of AV block through electrocardiography, and quantification of histological and immunofluorescence markers to analyze the AVCS. This protocol can be used to study mechanisms associated with AVCS injury repair and regeneration. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021). Reproducible ablation injury model of the mouse atrioventricular conduction system Different routes of tamoxifen induction among neonatal and adult mice Simplified instructions on neonatal and adult mice electrocardiography Detailed walkthrough of immunohistochemical techniques for AVCS tissue Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Damage to the atrioventricular conduction system (AVCS), the main electrical connection between the atrial and ventricular chambers, can result in a variety of cardiac conduction disorders. Here, we provide a protocol for selective damage of the mouse AVCS to study its response during injury. We describe tamoxifen-induced cellular ablation, detection of AV block through electrocardiography, and quantification of histological and immunofluorescence markers to analyze the AVCS. This protocol can be used to study mechanisms associated with AVCS injury repair and regeneration.
影响因子：
15.9
作者：
Wang, Lin;Bhakta, Minoti;Munshi, Nikhil V.
通讯作者：
Munshi, Nikhil V.
影响因子：
4.6
作者：
Munshi, Nikhil V.;McAnally, John;Olson, Eric N.
通讯作者：
Olson, Eric N.