ASS and SULT2A1 are Novel and Sensitive Biomarkers of Acute Hepatic Injury-A Comparative Study in Animal Models.
ASS and SULT2A1 are Novel and Sensitive Biomarkers of Acute Hepatic Injury-A Comparative Study in Animal Models.
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DOI：
10.4172/2167-0889.1000115
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发表时间：
2013-01
期刊：
影响因子：
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通讯作者：
V. Prima;M. Cao;S. Svetlov
中科院分区：
文献类型：
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作者：
V. Prima;M. Cao;S. Svetlov
与多发性创伤、内毒素休克/脓毒症和器官移植相关的肝和肾损伤是多器官衰竭的主要原因之一。开发新的敏感的生物标志物来检测肝脏和肾脏损伤的早期阶段对于有效诊断和治疗这些危及生命的疾病至关重要。以前，我们确定了几种肝脏蛋白，包括精氨琥珀酸合成酶（ASS）和磺基转移酶，它们在肝脏中降解，并在缺血/再灌注（I/R）损伤期间迅速释放到循环中。在这里，我们比较了新开发的夹心ELISA检测ASS和磺基转移酶亚型SULT 2A 1的灵敏度和特异性与标准临床肝脏和肾脏试验丙氨酸氨基转移酶（ALT）和天冬氨酸转氨酶（AST）在各种临床前急性损伤模型。我们的数据表明，ASS和SULT 2A 1在内毒素血症、缺血/再灌注（I/R）、化学和药物诱导的肝损伤中具有上级的肝肾健康评估特性，并且可能具有很高的临床应用价值。
Liver and kidney damage associated with polytrauma, endotoxic shock/sepsis, and organ transplantation, are among the leading causes of the multiple organ failure. Development of novel sensitive biomarkers that detect early stages of liver and kidney injury is vital for the effective diagnostics and treatment of these life-threatening conditions. Previously, we identified several hepatic proteins, including Argininosuccinate Synthase (ASS) and sulfotransferases which were degraded in the liver and rapidly released into circulation during Ischemia/Reperfusion (I/R) injury. Here we compared sensitivity and specificity of the newly developed sandwich ELISA assays for ASS and the sulfotransferase isoform SULT2A1 with the standard clinical liver and kidney tests Alanine Aminotransferase (ALT) and Aspartate Transaminase (AST) in various pre-clinical models of acute injury. Our data suggest that ASS and SULT2A1 have superior characteristics for liver and kidney health assessment in endotoxemia, Ischemia/Reperfusion (I/R), chemical and drug-induced liver injury and may be of high potential value for clinical applications.