Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo
Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo
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DOI：
10.1016/j.femsec.2005.03.017
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发表时间：
2005-10-01
影响因子：
4.2
通讯作者：
Yagi, O
中科院分区：
文献类型：
--
作者：
Limpiyakorn, T;Shinohara, Y;Yagi, O
研究了12个污水处理系统在3个不同季节的活性污泥中氨氧化细菌的分布情况。我们使用实时PCR定量，以揭示总细菌数和总氨氧化剂的数量，并使用特定的PCR，然后变性凝胶梯度电泳，克隆和测序的16 S rRNA基因分析氨氧化细菌群落。总细菌数和总氨氧化物数分别在1.6 × 10（12）-2.4 × 10（13）和1.0 × 10（9）-9.2 × 10（10）细胞/l（-1）范围内。氨氧化剂总数存在季节性变化，但氨氧化细菌群落却没有变化。寡养亚硝化单胞菌集群的成员被发现在所有的样品中，在这个集群内的大部分序列分组内的四个序列类型确定的两个。欧洲亚硝化单胞菌-运动亚硝化球菌、耐低温亚硝化单胞菌和未知亚硝化单胞菌群的成员仅发生在一个厌氧/缺氧/好氧（A2 O）系统中。亚硝化单胞菌集群的成员几乎只发生在与A2 O和厌氧/好氧系统。固体停留时间主要影响氨氧化细菌的总数，而溶解氧浓度主要影响氨氧化细胞的氨氧化活性。(C)2005年由Elsevier B. V.代表欧洲微生物学会联合会出版。
We investigated ammonia-oxidizing bacteria in activated sludge collected from 12 sewage treatment systems, whose ammonia removal and treatment processes differed, during three different seasons. We used real-time PCR quantification to reveal total bacterial numbers and total ammonia oxidizer numbers, and used specific PCR followed by denaturing gel gradient electrophoresis, cloning, and sequencing of 16S rRNA genes to analyze ammonia-oxidizing bacterial communities. Total bacterial numbers and total ammonia oxidizer numbers were in the range of 1.6 x 10(12)-2.4 x 10(13) and 1.0 x 10(9)-9.2 x 10(10) cells l(-1), respectively. Seasonal variation was observed in the total ammonia oxidizer numbers, but not in the ammonia-oxidizing bacterial communities. Members of the Nitrosomonas oligotropha cluster were found in all samples, and most sequences within this cluster grouped within two of the four sequence types identified. Members of the clusters of Nitrosomonas europaea-Nitrosococcus mobilis, Nitrosomonas cryotolerans, and unknown Nitrosomonas, occurred solely in one anaerobic/anoxic/aerobic (A2O) system. Members of the Nitrosonlonas communis cluster occurred almost exclusively in association with A2O and anaerobic/aerobic systems. Solid residence time mainly influenced the total numbers of ammonia-oxidizing bacteria, whereas dissolved oxygen concentration primarily affected the ammonia-oxidizing activity per ammonia oxidizer cell. (C) 2005 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.