Occurrence of different types of infectious hematopoietic necrosis virus in fish
Occurrence of different types of infectious hematopoietic necrosis virus in fish
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不同类型传染性造血坏死病毒在鱼类中的发生情况
DOI：
10.1128/aem.52.6.1353-1361.1986
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发表时间：
1986
影响因子：
4.4
通讯作者：
J. Leong
中科院分区：
文献类型：
--
作者：
Y. Hsu;H. Engelking;J. Leong
采用十二烷基硫酸钠- [35S]-蛋氨酸标记病毒聚丙烯酰胺凝胶电泳分析了太平洋西北地区71株感染性血细胞坏死病毒（IHNV）的病毒粒子蛋白谱。这一分析将这些病毒分离物分为四种或更多类型。1型病毒的特征是核衣壳蛋白，分子量约为40,500。2型和3型病毒的核衣壳蛋白分子量分别为42800和43250。2型病毒是最近哥伦比亚河下游鱼类中流行性乙型肝炎病毒的罪魁祸首。加州的IHNV分离株为3型，除了在萨克拉门托河的科尔曼孵化场从鱼类中分离出的一些外。这些科尔曼孵化场分离物属于4型病毒群，其特征是糖蛋白较大，分子量约为70,000。所有其他被检测的病毒的糖蛋白分子量为67000。“5型”病毒分离株被归类在一起，因为它们没有足够的区别，不能归类为单独的类型。这些发现有助于确定某一特定类型的病毒是某一地理区域的特征，并将感染该地区许多不同的鲑科鱼类，而从亲代鱼中分离出来的同一类型病毒是随后在后代中爆发疾病的原因。
The virion protein patterns of 71 isolates of infectious hematopoietic necrosis virus (IHNV) from the Pacific Northwest were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of [35S]-methionine-labeled virus. This analysis led to the classification of these virus isolates into four or more types. Type 1 virus was characterized by a nucleocapsid protein with an approximate molecular weight of 40,500. Type 2 and type 3 viruses have nucleocapsid proteins with molecular weights of 42,800 and 43,250, respectively. Type 2 virus was responsible for the recent epizootics of IHNV among fish in the lower Columbia River. The California IHNV isolates were type 3 with the exception of some of those isolated from fish at the Coleman Hatchery on the Sacramento River. These Coleman Hatchery isolates belonged to a type 4 virus group characterized by a larger glycoprotein of approximately 70,000 molecular weight. All other viruses examined had glycoproteins of 67,000 molecular weight. The "type 5" virus isolates were grouped together because they were not sufficiently distinct to warrant classification into a separate type. These findings have been useful in determining that a particular virus type is characteristic for a geographic area and will infect many different salmonid species in that area and the same type isolated from parental fish is responsible for the subsequent outbreak of the diseases in progeny.