Morrenain b I, a Papain-Like Endopeptidase from the Latex of Morrenia brachystephana Griseb. (Asclepiadaceae)
Morrenain b I, a Papain-Like Endopeptidase from the Latex of Morrenia brachystephana Griseb. (Asclepiadaceae)
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DOI：
10.1023/a:1023059525861
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
Sandra Vairo Cavalli;M. Arribére;A. Cortadi;N. Caffini;N. Priolo
中科院分区：
文献类型：
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作者：
Sandra Vairo Cavalli;M. Arribére;A. Cortadi;N. Caffini;N. Priolo
从短柄Morrenia brachystephanaGrise B的茎和叶柄中分离纯化得到一种新的半胱氨酸内肽酶（morrenain B I）。（萝摩科）。Morrenain B I是胶乳中的次要蛋白水解组分，但比Morrenain B II显示出更高的比活性，Morrenain B II是主要活性组分。这两种酶表现出相似的pH值和分子量，但动力学参数和N-末端序列是相当不同的，表明它们是不同的酶，而不是不同形式的相同的酶。
A new cysteine endopeptidase (morrenain b I) has been purified and characterized from the latex of stems and petiols ofMorrenia brachystephanaGriseb. (Asclepiadaceae). Morrenain b I was the minor proteolytic component in the latex but showed higher specific activity than morrenain b II, which was the main active fraction. Both enzymes showed similar pH profiles and molecular masses, but kinetic parameters and N-terminal sequences were quite distinct, demonstrating that they are different enzymes instead of different forms of the same enzyme.