Effect of mono-ethylhexyl phthalate on MA-10 Leydig tumor cells
Effect of mono-ethylhexyl phthalate on MA-10 Leydig tumor cells
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DOI：
10.1016/s0890-6238(01)00110-1
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发表时间：
2001-03-01
影响因子：
3.3
通讯作者：
Papadopoulos, V
中科院分区：
文献类型：
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作者：
Dees, JH;Gazouli, M;Papadopoulos, V
我们研究了邻苯二甲酸单乙基己酯（MEHP）对MA-IO Leydig肿瘤细胞结构和功能的影响。将细胞暴露于不同浓度的MEHP 24小时，然后用饱和浓度的hCG刺激2.5小时。低浓度MEHP中度抑制孕酮生成、细胞活力和蛋白质含量，高浓度MEHP严重抑制孕酮生成、细胞活力和蛋白质含量。电子显微镜检查显示，在MEHP处理的细胞，随着MEHP浓度的增加，严重程度增加的各种变化。在MEHP处理的细胞中，脂滴受到了深刻的影响，并且观察到形态学证据表明，在孕酮合成停止的大约同一时间，脂质储存滴的代谢停止。形态测定研究表明，MEHP浓度为10（-6）至10（-3）M时，脂滴数量似乎比对照水平增加了2.5倍，而线粒体体积分数降低。这些结果表明，间质细胞中的MEHP可能作为线粒体毒物和脂质代谢破坏。(C)2001 Elsevier Science Inc. All rights reserved.
We examined the effect of mono-ethylhexyl phthalate (MEHP) on MA-IO Leydig tumor cell structure and function. Cells were exposed to various concentrations of MEHP for 24 h and then stimulated with saturating concentrations of hCG for 2.5 h. Progesterone production, cell viability, and protein content were moderately inhibited by low concentrations and severely inhibited by high concentrations of MEHP. Electron microscopy showed a variety of alterations in the MEHP-treated cells, increasing in severity with increasing concentrations of MEHP. Lipid droplets were profoundly affected in the cells treated with MEHP and morphologic evidence that metabolism of lipid storage droplets ceases at approximately the same time progesterone synthesis stops was seen. Morphometric studies indicated that the number of lipid droplets appeared to be increased 2.5-fold over control levels at MEHP concentrations of 10(-6) to 10(-3) M whereas mitochondrial volume fraction decreased. These results suggest that MEHP in Leydig cells may act as a mitochondrial toxicant and lipid metabolism disrupter. (C) 2001 Elsevier Science Inc. All rights reserved.