Identification of Spodoptera litura multicapsid nucleopolyhedrovirus ORF97, a novel protein associated with envelope of occlusion-derived virus
Identification of Spodoptera litura multicapsid nucleopolyhedrovirus ORF97, a novel protein associated with envelope of occlusion-derived virus
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斜纹夜蛾多衣壳核多角体病毒 ORF97 的鉴定，一种与包膜衍生病毒包膜相关的新型蛋白
DOI：
10.1007/s11262-005-5848-x
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发表时间：
2006-02-01
期刊：
影响因子：
1.6
通讯作者：
Pang, Y
中科院分区：
文献类型：
--
作者：
Chen, WC;Li, ZF;Pang, Y
Open reading frame (ORF) 97 of Spodoptera litura multicapsid nucleopolyhedrovirus (Splt97) is a member of 11k gene family (InterPro database accession number: IPR009313) in insect viruses, potentially encoding 112 amino acids with a predicted molecular weight of 13.2 kDa. Sequence analysis indicated that Splt97 has 8-50% amino acid identity with the homologues of other known baculoviruses including Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) ORF108. Splt97 transcription in SpltMNPV-infected Spli-221 cells was detected from 6 to 96 h postinfection (p.i.) and the mRNA start site was mapped within a consensus baculovirus early promoter motif (ACATT). Time course of Splt97 expression in SpltMNPV-infected cells showed that Splt97 was expressed from 48 through 96 h p.i. as a 13 kDa protein. Western blot analysis revealed that Splt97 was present in occlusion-derived virus (ODV). Furthermore, when ODV was fractionated into nucleocapsid and envelope components, Splt97 was shown to be present only in the envelope. These results suggested that Splt97 was a baculovirus ODV-associated protein.
