Critical role of sensitized serum in rejection of allogeneic bone marrow cells.
Critical role of sensitized serum in rejection of allogeneic bone marrow cells.
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DOI：
10.4274/tjh.2012.0213
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发表时间：
2014-09-05
期刊：
影响因子：
--
通讯作者：
Xu HG
中科院分区：
文献类型：
--
作者：
Xu LH;Fang JP;Weng WJ;Xu HG
目的：体液免疫在实体器官移植中有明确的作用，但对体液免疫与造血干细胞移植的关系知之甚少。本研究旨在调查这种关系。 材料与方法：采用同种异体脾细胞输注建立致敏小鼠模型，获得致敏血清。致敏血清与同种异体骨髓细胞体外孵育，采用补体依赖细胞毒法评价其细胞毒作用。小鼠移植同种异体骨髓细胞与致敏血清孵育后致死辐射。通过造血恢复和嵌合体分析来测定植入。此外，小鼠在移植前1天接受致敏血清的被动转移。骨髓移植后每天对死亡率进行评分。 结果如下：体外实验表明，致敏血清能够通过补体依赖的细胞毒性途径损伤同种异体BMCs。动物实验表明，骨髓基质细胞与致敏血清孵育不能挽救小鼠的致死辐射。移植实验表明，同种异体骨髓细胞与致敏血清孵育后，随着时间的推移，在受体体内出现排斥反应。此外，小鼠在移植前因转移致敏血清而死于骨髓移植排斥。 结论：综上所述，我们的结果表明，致敏血清在造血干细胞移植的移植排斥反应中起着关键作用。
Objective: Humoral immunity has been clearly implicated in solid organ transplantation, but little is known about the relationship between humoral immunity and hematopoietic stem cell transplantation. This study was designed to investigate that relationship. Materials and Methods: Sensitized serum was obtained from a sensitized murine model established by allogeneic splenocyte transfusion. Sensitized serum was incubated with allogeneic bone marrow cells (BMCs) in vitro and the cytotoxicity was evaluated by the complement-dependent cytotoxicity method. Mice were transplanted with allogeneic BMCs incubated with sensitized serum after lethal irradiation. The engraftment was assayed by hematopoietic recovery and chimera analysis. Moreover, mice received passive transfer of sensitized serum 1 day prior to transplantation. Mortality was scored daily after bone marrow transplantation. Results: The in vitro experiments showed that sensitized serum was capable of impairing allogeneic BMCs through the complement-dependent cytotoxicity pathway. The animal studies showed that BMCs incubated with sensitized serum failed to rescue mice from lethal irradiation. The engraftment assay showed that the allogeneic BMCs incubated with sensitized serum were rejected with time in the recipients. Furthermore, the mice died of marrow graft rejection by transfer of sensitized serum prior to transplantation. Conclusion: Taken together, our results indicated that sensitized serum played a critical role in graft rejection during hematopoietic stem cell transplantation.
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影响因子：
6.2
作者：
Nagata, Shigeyuki;Okano, Shinji;Sueishi, Katsuo
通讯作者：
Sueishi, Katsuo
影响因子：
20.3
作者：
Spellman, Stephen;Bray, Robert;Anasetti, Claudio
通讯作者：
Anasetti, Claudio
影响因子：
20.3
作者：
Taylor, Patricia A.;Ehrhardt, Michael J.;Blazarl, Bruce R.
通讯作者：
Blazarl, Bruce R.
影响因子：
20.3
作者：
SEMPLE, JW;SPECK, ER;FREEDMAN, J
通讯作者：
FREEDMAN, J
DOI：
10.1111/j.1600-6143.2011.03593.x
发表时间：
2011-09
期刊：
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
影响因子：
--
作者：
O'Leary JG;Kaneku H;Susskind BM;Jennings LW;Neri MA;Davis GL;Klintmalm GB;Terasaki PI
通讯作者：
Terasaki PI