TRA1 : a locus responsible for controlling Agrobacterium -mediated transformability in barley
TRA1 : a locus responsible for controlling Agrobacterium -mediated transformability in barley
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TRA1：负责控制大麦中农杆菌介导的转化性的基因座
DOI：
10.1101/2019.12.19.882274
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Orman-Ligeza B
中科院分区：
文献类型：
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作者：
Orman-Ligeza B
在大麦（Hordeum vulcanum L.）中，农杆菌介导的转化效率高度依赖于基因型，很少有品种适合转化。Golden Promise是最广泛用于大麦转化的品种，发育中的胚是最常见的供体组织。我们测试了具有异常大胚的大麦突变体是否或多或少适合于转化，并发现突变体M1460具有与Golden Promise相似的转化效率。M1460的大胚胎表型是由于LYS 3位点的突变造成的。在相同的LYS3位点上，还有三个其他的大麦品系具有独立的突变，其中之一，Risø1508具有与M1460相同的错义突变。然而，除M1460外，其余3个突变体均不能转化，表明TRA1不是LYS3，而是M1460所特有的另一个基因座。为了鉴定TRA1，我们通过将M1460与对转化不敏感的品种Optic杂交来产生分离群体。在与Optic进行四轮回交后，对植物进行基因分型，并测试其后代的可转化性。一些后代系可在与亲本M1460相似的高效率下转化，而一些后代系不可转化，如Optic。染色体2H上的一个区域遗传自M1460，仅存在于可转化系中。我们推测该区域的225个基因之一是TRA1。
In barley (Hordeum vulgareL.),Agrobacterium-mediated transformation efficiency is highly dependent on genotype with very few cultivars being amenable to transformation. Golden Promise is the cultivar most widely used for barley transformation and developing embryos are the most common donor tissue. We tested whether barley mutants with abnormally large embryos were more or less amenable to transformation and discovered that mutant M1460 had a transformation efficiency similar to that of Golden Promise. The large-embryo phenotype of M1460 is due to mutation at theLYS3locus. There are three other barley lines with independent mutations at the sameLYS3locus, and one of these, Risø1508 has an identical missense mutation to that in M1460. However, none of thelys3mutants except M1460 were transformable showing that the locus responsible for transformation efficiency,TRA1, was notLYS3but another locus unique to M1460. To identifyTRA1, we generated a segregating population by crossing M1460 to the cultivar Optic, which is recalcitrant to transformation. After four rounds of backcrossing to Optic, plants were genotyped and their progeny were tested for transformability. Some of the progeny lines were transformable at high efficiencies similar to those seen for the parent M1460 and some were not transformable, like Optic. A region on chromosome 2H inherited from M1460 is present in transformable lines only. We propose that one of the 225 genes in this region isTRA1.