Rapid gas chromatographic separation of amino acid enantiomers with a novel chiral stationary phase.
Rapid gas chromatographic separation of amino acid enantiomers with a novel chiral stationary phase.
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DOI：
10.1093/chromsci/15.5.174
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发表时间：
1977-05
影响因子：
1.3
通讯作者：
H. Frank;G. Nicholson;E. Bayer
中科院分区：
文献类型：
--
作者：
H. Frank;G. Nicholson;E. Bayer
使用新型的聚硅氧烷作为固定相携带手性基团，使大多数氨基酸对映体的分离在一个更短的时间比以往任何时候都报道。这种类型的相表现出非常低的挥发性和高热稳定性，并且可以在至少175 ℃的温度下用开放式管柱进行常规分析。大多数蛋白质氨基酸在90至175摄氏度之间的温度程序中分离，从而避免了多次注射的需要。分离因子略低于含有L-缬氨酸叔丁基酰胺基团的其他二酰胺相，但足以分离几乎所有蛋白质氨基酸对映体。
The use of novel polysiloxanes as stationary phase carrying chiral groups enables the separation of most amino acid enantiomers in a much shorter time than ever reported previously. Phases of this type exhibit very low volatility and high thermal stability and may be used in routine analysis with open tubular columns ant temperatures of at least 175 degrees C. Most protein amino acids are separated in a temperature program between 90 and 175 degrees C, thus obviating the need for multiple injections. Resolution factors are somewhat lower than those of other diamide phases containing the L-valine t-butyl-amide group, but are sufficient for resolution of almost all protein amino acid enantiomers.