Proteomic profiling of 24-epibrassinolide-induced chilling tolerance in harvested banana fruit
Proteomic profiling of 24-epibrassinolide-induced chilling tolerance in harvested banana fruit
复制标题
24-表油菜素内酯诱导收获香蕉果实耐冷性的蛋白质组学分析
DOI：
10.1016/j.jprot.2018.05.011
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发表时间：
2018-09-15
影响因子：
3.3
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
--
作者：
Li, Taotao;Yun, Ze;Jiang, Yueming
研究了24-表油菜素内酯（EBR）诱导香蕉果实耐冷性的机制。结果表明，EBR预处理显著抑制了香蕉果实在8 ℃冷藏12 d后冷害的发生，降低了香蕉果实的冷害指数。生理测定结果表明，EBR处理降低了相对电解质渗漏率和丙二醛（MDA）含量，提高了叶绿素荧光（Fv/Fm）、可溶性固形物（TSS）和TSS/可滴定酸比值。此外，采用凝胶蛋白质组学技术结合MALDI-TOF-TOF MS和LC-ESI-MS/MS分析，研究了香蕉果实对EBR和低温处理的差异蛋白质积累。成功鉴定了55个蛋白质点。EBR诱导表达的蛋白质主要与能量合成、胁迫反应和细胞壁修饰有关。相反，EBR处理下调了参与蛋白质降解和能量消耗的蛋白质。以上结果表明，EBR处理可以通过促进蛋白质的合成和抑制蛋白质的降解，提高香蕉果实的防御能力，促进能量的合成和利用，维持蛋白质的功能，从而有助于提高香蕉果实的耐冷性。为了加深对香蕉果实冷害的认识，我们报道了24-表油菜素（EBR）对香蕉果实在8 ℃贮藏时冷害的影响。这是首次对香蕉果实在低温下对EBR反应的蛋白质组学研究。EBR预处理显著降低了采收香蕉果实的CI。成功鉴定了55个蛋白质点。EBR诱导表达的蛋白质主要与能量合成、胁迫反应和细胞壁修饰有关。相反，参与蛋白质降解和能量消耗的蛋白质被下调。这些结果表明，外源EBR处理可以增强防御能力，维持高能量状态。同时，EBR处理通过促进蛋白质的生物合成和抑制蛋白质的降解来维持蛋白质的功能。这些结果有助于我们理解EBR处理诱导香蕉果实耐冷性的分子机制，并拓宽了对采后香蕉果实耐冷性机理的认识。
The mechanism of 24-epibrassinolide (EBR)-induced chilling tolerance in harvested banana fruit was investigated. Results showed that EBR pretreatment remarkably suppressed the development of chilling injury (CI) in harvested banana fruit during 12 days of cold storage at 8 degrees C, as indicated by lower CI index in treated fruit. Physiological measurements exhibited that EBR treatment reduced the relative electrolyte leakage and malondialdehyde (MDA) content while increased the chlorophyll fluorescence (Fv/Fm), total soluble solids (TSS) and ratio of TSS and titratable acidity. Furthermore, the differentially accumulated proteins of banana fruit in response to EBR and cold treatment were investigated by employing gel-based proteomic in combination with MALDI-TOF-TOF MS and LC-ESI-MS/MS analyses. There were fifty five protein spots to be successfully identified. Notably, most of up-regulated proteins by EBR treatment were related to energy biosynthesis, stress response and cell wall modification. In contrast, proteins involved in protein degradation and energy consumption were down-regulated by EBR treatment. These results suggest that EBR treatment could enhance the defense ability, promote the synthesis and utilization of energy, as well as maintain the protein function via enhancing protein biosynthesis and inhibiting protein degradation, consequently contributing to improvement of cold tolerance in harvested banana fruit.Significance: To extend our understanding of chilling injury (CI) of harvested banana fruit, we reported the effect of 24-epibrassinolide (EBR) on CI of banana fruit when stored at 8 degrees C. It was the first report on the comprehensive proteomic analysis of banana fruit in response to EBR treatment at low temperature. EBR pretreatment significantly reduced CI in harvested banana fruit. Fifty five protein spots were successfully identified. Notably, the most of up-regulated proteins by EBR treatment were related to energy biosynthesis, stress response and cell wall modification. In contrast, proteins involved in protein degradation and energy consumption were down regulated. These results suggest that exogenous EBR treatment could enhance the defense ability and maintain high energy status. Meanwhile, EBR treatment maintained protein function via enhancing protein biosynthesis and inhibiting protein degradation. These results may help us to understand the molecular mechanism of the chilling tolerance induced by EBR treatment and broaden the current knowledge of the mechanism of CI of harvested banana fruit.