Comprehensive multilevel in vivo and in vitro analysis of heart rate fluctuations in mice by ECG telemetry and electrophysiology
Comprehensive multilevel in vivo and in vitro analysis of heart rate fluctuations in mice by ECG telemetry and electrophysiology
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DOI：
10.1038/nprot.2015.139
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发表时间：
2016-01-01
期刊：
影响因子：
14.8
通讯作者：
Wahl-Schott, Christian
中科院分区：
文献类型：
--
作者：
Fenske, Stefanie;Proebstle, Rasmus;Wahl-Schott, Christian
正常心跳在平均值附近略有波动；这种现象称为生理心率变异性（HRV）。众所周知，HRV 改变是心源性猝死的危险因素。基因小鼠模型的出现使得通过实验剖析 HRV 病理变化的机制及其与心源性猝死的关系成为可能。在这里，我们提供了一个协议，可以对心率 (HR) 波动进行全面的多级分析。该协议包括一组技术，包括完整窦房网络制剂或分离的单个窦房结 (SANSANSAN) 细胞的体内遥测和体外电生理学。体外制备可在数小时内完成，数据采集可在 1 d 内完成。体内遥测 ECECG 手术需要 1 小时，数据采集和分析需要几周时间。该协议对研究心血管生理学和心源性猝死病理生理学的研究人员感兴趣。
The normal heartbeat slightly fluctuates around a mean value; this phenomenon is called physiological heart rate variability (HRV). It is well known that altered HRV is a risk factor for sudden cardiac death. The availability of genetic mouse models makes it possible to experimentally dissect the mechanism of pathological changes in HRV and its relation to sudden cardiac death. Here we provide a protocol that allows for a comprehensive multilevel analysis of heart rate (HR) fluctuations. The protocol comprises a set of techniques that include in vivo telemetry and in vitro electrophysiology of intact sinoatrial network preparations or isolated single sinoatrial node (SANSANSAN) cells. In vitro preparations can be completed within a few hours, with data acquisition within 1 d. In vivo telemetric ECECG requires 1 h for surgery and several weeks for data acquisition and analysis. This protocol is of interest to researchers investigating cardiovascular physiology and the pathophysiology of sudden cardiac death.