Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway
Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway
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DOI：
10.1104/pp.103.022244
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发表时间：
2003-07-01
期刊：
影响因子：
7.4
通讯作者：
Good, AG
中科院分区：
文献类型：
--
作者：
Ismond, KP;Dolferus, R;Good, AG
我们利用相应基因（PDC1、PDC2 和 ADHI）的有义和反义过表达来操纵拟南芥中酒精发酵途径丙酮酸脱羧酶（PDC）和乙醇脱氢酶（ADH）的酶活性水平。分析转基因植物的发酵水平并评估缺氧存活的变化。拟南芥 PDC1 或 PDC2 的过度表达可提高植物的存活率。相比之下，拟南芥 ADH1 的过度表达对洪水生存没有影响。这些结果支持 PDC 作为乙醇发酵控制步骤的作用。尽管 ADH1 无效突变体降低了缺氧存活率，但试图降低 PDC 活性水平以观察对植物存活的影响，但取得的成功有限。洪水生存数据和代谢物分析的结合可以识别关键的代谢通量点。该信息可用于设计转基因策略以提高植物的缺氧耐受性。
We manipulated the enzyme activity levels of the alcohol fermentation pathway, pyruvate decarboxylase (PDC), and alcohol dehydrogenase (ADH) in Arabidopsis using sense and antisense overexpression of the corresponding genes (PDC1, PDC2, and ADHI). Transgenic plants were analyzed for levels of fermentation and evaluated for changes in hypoxic survival. Overexpression of either Arabidopsis PDC1 or PDC2 resulted in improved plant survival. In contrast, overexpression of Arabidopsis ADH1 had no effect on flooding survival. These results support the role of PDC as the control step in ethanol fermentation. Although ADH1 null mutants had decreased hypoxic survival, attempts to reduce the level of PDC activity enough to see an effect on plant survival met with limited success. The combination of flooding survival data and metabolite analysis allows identification of critical metabolic flux points. This information can be used to design transgenic strategies to improve hypoxic tolerance in plants.