Novel neuropeptide YY2-like receptor subtype in zebrafish and frogs supports early vertebrate chromosome duplications
Novel neuropeptide YY2-like receptor subtype in zebrafish and frogs supports early vertebrate chromosome duplications
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DOI：
10.1007/s00239-003-2529-z
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发表时间：
2004-01-01
影响因子：
3.9
通讯作者：
Larhammar, D
中科院分区：
文献类型：
--
作者：
Fredriksson, R;Larson, ET;Larhammar, D
Y受体包括一个以神经肽Y家族肽为内源性配体的g蛋白偶联受体家族。Y受体家族在哺乳动物中有5个成员，进化数据表明，它在脊椎动物进化早期发生的两次基因组复制中多样化。如果这个理论是正确的，它允许额外的家庭成员在场。我们在这里描述的克隆，药理学特征，组织分布和染色体定位的一个新的亚型y受体家族，命名为Y7，从斑马鱼。我们还介绍了虹鳟和两种两栖动物的Y7序列。新受体与Y2最相似，同源性为51-54%。由于Y2也从这些物种中克隆出来，显然有两个独立的Y2亚家族基因。斑马鱼的染色体定位支持Y7起源于早期脊椎动物的染色体复制。Y7可能在哺乳动物的谱系中丢失了。斑马鱼Y7受体的药理学特征与哺乳动物的Y2不同，因为它不以高亲和力结合NPY的短片段。Y7受体支持早期脊椎动物基因组复制理论，并表明Y受体家族是这些早期基因组复制的结果。
The Y receptors comprise a family of G-protein coupled receptors with neuropeptide Y-family peptides as endogenous ligands. The Y receptor family has five members in mammals and evolutionary data suggest that it diversified in the two genome duplications proposed to have occurred early in vertebrate evolution. If this theory holds true, it allows for additional family members to be present. We describe here the cloning, pharmacological characterization, tissue distribution, and chromosomal localization of a novel subtype of the Y-receptor family, named Y7, from the zebrafish. We also present Y7 sequences from rainbow trout and two amphibians. The new receptor is most similar to Y2 with 51-54% identity. As Y2 has also been cloned from some of these species, there clearly are two separate Y2-subfamily genes. Chromosomal mapping in zebrafish supports origin of Y7 as a duplicate of Y2 by chromosome duplication in an early vertebrate. Y7 has probably been lost in the lineage leading to mammals. The pharmacological profile of the zebrafish Y7 receptor is different from mammalian Y2, as it does not bind short fragments of NPY with a high affinity. The Y7 receptor supports the theory of early vertebrate genome duplications and suggests that the Y family of receptors is a result of these early genome duplications.