Rapid appearance of M cells after microbial challenge is restricted at the periphery of the follicle-associated epithelium of Peyer's patch.
Rapid appearance of M cells after microbial challenge is restricted at the periphery of the follicle-associated epithelium of Peyer's patch.
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微生物攻击后，M 细胞的快速出现受到派尔氏淋巴结滤泡相关上皮周围的限制。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Claudio Nicoletti
中科院分区：
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作者：
C. Borghesi;Michael J. Taussig;Claudio Nicoletti
肠道滤泡相关上皮（FAE）内的M细胞通过将抗原转运至肠道淋巴组织，在启动粘膜免疫应答中发挥核心作用。我们以前已经证明，滴注到肠道的非肠道微生物，肺炎链球菌R36 a，是一个很好的实验模型，调查的高度动态性质的FAE在微生物的挑战。在本研究中，S.将肺炎链球菌引入兔回肠袢中，每个回肠袢含有派尔集合淋巴结（PP），并且在短时间（1-3小时）后通过FAE的不同区域中的形态学和功能特征来评估M细胞的数量。我们报道，在暴露于S后1小时，在FAE的外周检测到M细胞的数量显著增加，但在顶端区域没有。肺炎。此外，该实验的一个变体使我们能够确定M细胞数量的增加导致FAE从肠腔向肠淋巴系统运输乳胶荧光微球（0.5 μ m）的能力提高，所述乳胶荧光微球对兔M细胞具有高度特异性。在这些动物中，乳糜池被插管，并且在用肺炎球菌刺激后将微粒引入肠袢中。然后通过流式细胞仪计数到达淋巴的微粒。我们解释这些结果表明，只有位于周边的FAE肠上皮细胞转化为完全运作的M细胞通过某些微生物的相互作用和肠上皮细胞进行这种转换的能力可能取决于他们的分化阶段。
M cells within the follicle-associated epithelium (FAE) of the gut play a central role in the initiation of mucosal immune responses by transporting antigens to the intestinal lymphoid tissue. We have previously demonstrated that the instillation into the gut of a nonenteric microorganism, Streptococcus pneumoniae R36a, is an excellent experimental model to investigate the highly dynamic nature of the FAE in response to microbial challenge. In the present study, S. pneumoniae was introduced into rabbit ileal loops, each one containing a Peyer's patch (PP), and the number of M cells was assessed by morphological and functional characteristics in different areas of the FAE after a short time (1-3 hours). We report that a marked increase in the number of M cells was detected in the periphery, but not in the apical area, of the FAE as early as 1 hour after exposure to S. pneumoniae. Furthermore, a variant of this experiment enabled us to establish that the increased numbers of M cells led to an improved capability of the FAE to transport latex fluorescent microspheres (0.5 microm), highly specific to rabbit M cells, from the gut lumen to the intestinal lymphatic system. In these animals the cisterna chyli was cannulated, and the microparticles were introduced into the intestinal loops after stimulation with pneumococci. The microparticles reaching the lymph were then counted by flow cytometer. We interpreted these results as showing that only enterocytes located within the periphery of the FAE are converted to fully operational M cells by certain microbial interaction and the ability of enterocytes to undergo this conversion may depend on their stage of differentiation.