Promising interaction between nanoencapsulated lutein with low molecular weight chitosan: Characterization and bioavailability of lutein in vitro and in vivo
Promising interaction between nanoencapsulated lutein with low molecular weight chitosan: Characterization and bioavailability of lutein in vitro and in vivo
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DOI：
10.1016/j.foodchem.2013.02.108
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发表时间：
2013-11-01
期刊：
影响因子：
8.8
通讯作者：
Baskaran, Vallikannan
中科院分区：
文献类型：
--
作者：
Arunkumar, Ranganathan;Prashanth, Keelara Veerappa Harish;Baskaran, Vallikannan
本研究旨在开发含有叶黄素的水溶性低分子壳聚糖纳米胶囊，以提高其生物利用度。以叶黄素为对照，制备了叶黄素-LMWC纳米胶囊，并对其进行了表征和体内外生物利用度研究。原子力显微镜证实其粒径在80~600 nm之间。H-1和C-13核磁共振研究表明，水分子以可逆的弱键将叶黄素固定在纳米颗粒的LMWC链之间。叶黄素(200 MM)的体外生物利用度显示，叶黄素-LMWC纳米胶囊的生物利用度显著高于对照(27.7%)。饲喂纳米叶黄素的小鼠餐后血浆(54.5%)、肝脏(53.9%)和眼睛(62.8%)中的叶黄素水平均高于对照组。LMWC可作为提高叶黄素生物利用度的新型载体，在食品和医药应用中可作为较好的膳食化合物。(C)2013爱思唯尔有限公司。保留所有权利。
This study aims to develop water-soluble low molecular weight chitosan (LMWC) nanoencapsules with lutein to improve its bioavailability. Lutein-LMWC nanoencapsules were prepared, characterized and bioavailability was studied in vitro and in vivo with lutein in mixed micelles (control). The particle size ranged between 80-600 nm, which was confirmed by Atomic Force Microscope. The interaction between LMWC and lutein in nanocencapsules by H-1 and C-13 NMR showed the essentiality of water molecules to hold the lutein between LMWC chains of nanoparticle with a reversible weak bond. Bioavailability of lutein (200 mu M) in vitro showed that lutein-LMWC nanoencapsules was significantly higher (27.7%) than control. Postprandial lutein level in the plasma (54.5%), liver (53.9%) and eyes (62.8%) of mice fed on nanoencapsulated lutein were higher than the control. LMWC may serve as novel carrier for enhancing the lutein bioavailability and can be suggested as the better dietary compound in food and pharmaceutical applications. (C) 2013 Elsevier Ltd. All rights reserved.