Effect of waterborne zinc exposure on metal accumulation, enzymatic activities and histology of Synechogobius hasta.
Effect of waterborne zinc exposure on metal accumulation, enzymatic activities and histology of Synechogobius hasta.
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DOI：
10.1016/j.ecoenv.2011.06.018
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发表时间：
2011-10
影响因子：
6.8
通讯作者：
J-L Zheng;Z. Luo;Q-L Chen;X. Liu;C-X Liu;Y. Zhao;Y. Gong
中科院分区：
文献类型：
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作者：
J-L Zheng;Z. Luo;Q-L Chen;X. Liu;C-X Liu;Y. Zhao;Y. Gong
研究了0.35、9.7和19.2 mg/L锌分别于0、4、8、12天对虎尾鱼体内金属积累、抗氧化酶反应、肝脏代谢和组织学的影响。水中锌暴露显著降低肝体指数、肝脏脂肪含量和脂肪肝发生率，增加肝脏中锌、铁、锰含量，降低肝脏中铜、钙含量，增加肌肉锌含量。水锌暴露还显著影响肝和脾中与抗氧化反应有关的酶活性(超氧化物歧化酶、过氧化氢酶、谷胱甘肽-S转移酶、丙二醛)，改变肝脏中间体酶活性(琥珀酸脱氢酶、苹果酸脱氢酶、乳酸脱氢酶、脂蛋白脂肪酶、肝脂酶)，破坏鳃和脾的组织结构，减少空泡化的肝细胞。因此，我们的研究首次证明，水中锌暴露可以减少沙门氏菌的脂肪肝综合征。
The present study was conducted to determine the metal accumulation, antioxidant enzymatic response, hepatic intermediary metabolism and histological changes in Synechogobius hasta exposed to 0.35 (control), 9.7 and 19.2mg/L Zn, respectively, on the 0, 4th, 8th and 12th day. Waterborne Zn exposure significantly reduced hepatosomatic index, hepatic lipid contents and fatty liver occurrence rate, increased Zn, Fe and Mn contents and reduced the contents of Cu and Ca in liver, and increased muscle Zn content. Waterborne Zn exposure also significantly influenced enzymatic activities involved in antioxidant responses (superoxide dismutase, catalase, glutathione-S-transferase, malondialdehyde) in liver and spleen, and changed hepatic intermediary enzymatic activities (succinate dehydrogenase, malic dehydrogenase, lactate dehydrogenase, lipoprotein lipase, hepatic lipase), impaired the histological structure of the gill and spleen, and reduced vacuolated hepatocytes. Thus, our study demonstrated for the first time that waterborne Zn exposure could reduce fatty liver syndrome in S. hasta.