Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting.
Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting.
复制标题
紫外熔融核酸纳米颗粒杂交的热力学表征。
DOI：
10.1007/978-1-0716-3417-2_9
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Khisamutdinov,EmilF
中科院分区：
文献类型：
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作者：
Teter,Megan;Brumett,Ross;Coffman,Abigail;Khisamutdinov,EmilF
核酸纳米技术的进步催生了由DNA、RNA、化学修饰的RNA链及其混合物制成的各种设计精美的结构络合物。NA纳米颗粒(NANP)的结构性质通常决定并显著影响生物功能；因此，提取关于不同结构形式的相对稳定性的信息至关重要。充分的稳定性评估需要热力学参数的知识，这些参数可以使用传统的UV熔融技术进行经验推导。本章的重点是描述以DNA 12碱基对(BP)双链形成为例的NANPS络合热力学数据的计算方法。
The advances in nucleic acid nanotechnology have given rise to various elegantly designed structural complexes fabricated from DNA, RNA, chemically modified RNA strands, and their mixtures. The structural properties of NA nanoparticles (NANP) generally dictate and significantly impact biological function; and thus, it is critical to extract information regarding relative stabilities of the different structural forms. The adequate stability assessment requires knowledge of thermodynamic parameters that can be empirically derived using conventional UV-melting technique. The focus of this chapter is to describe methodology to evaluate thermodynamic data of NANPs complexation based on DNA 12 base-pair (bp) duplex formation as an example.