Molecular assays of pollen use consistently reflect pollinator visitation patterns in a system of flowering plants
Molecular assays of pollen use consistently reflect pollinator visitation patterns in a system of flowering plants
复制标题
花粉使用的分子测定一致地反映了开花植物系统中的传粉者访问模式
DOI：
10.1111/1755-0998.13468
复制
发表时间：
2021
影响因子：
7.7
通讯作者：
Toews, David P. L.
中科院分区：
文献类型：
--
作者：
James, Aubrie R. M.;Geber, Monica A.;Toews, David P. L.
确定传粉者如何访问植物与它们如何携带和传递花粉是传粉生态学中正在进行的项目。目前用于识别蜜蜂携带的花粉的工具在用于生态推断时有不同的优点和缺点。在这项研究中，我们使用三种方法来更好地了解系统的同属，共花植物属Clarkia和他们的蜜蜂传粉者：观察植物传粉者接触的领域，和两种不同的分子方法来估计的相对丰度eachClarkiaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplaplapl我们使用这些方法来调查，如果观察植物传粉者接触在现场对应的花粉蜜蜂携带;如果个别蜜蜂carryClarkiapollens在可预测的方式，根据以前的知识，他们的觅食行为;以及如何理解植物传粉者的相互作用的三种方法不同。我们发现，植物传粉者接触的观察一般预测蜜蜂觅食时携带的花粉，使用三种不同方法的网络拓扑结构在统计上是无法区分的。分子花粉分析的结果还表明，虽然蜜蜂可以同时携带多种Clarkia，但它们通常携带一种花粉。我们的工作有助于越来越多的文献，旨在解决传粉者如何使用花卉资源。我们建议我们的新的相对扩增子定量方法作为另一种工具，在发展中的分子生态学和授粉生物学工具箱。
Determining how pollinators visit plants vs. how they carry and transfer pollen is an ongoing project in pollination ecology. The current tools for identifying the pollens that bees carry have different strengths and weaknesses when used for ecological inference. In this study we use three methods to better understand a system of congeneric, coflowering plants in the genusClarkiaand their bee pollinators: observations of plant–pollinator contact in the field, and two different molecular methods to estimate the relative abundance of eachClarkiapollen in samples collected from pollinators. We use these methods to investigate if observations of plant–pollinator contact in the field correspond to the pollen bees carry; if individual bees carryClarkiapollens in predictable ways, based on previous knowledge of their foraging behaviors; and how the three approaches differ for understanding plant–pollinator interactions. We find that observations of plant–pollinator contact are generally predictive of the pollens that bees carry while foraging, and network topologies using the three different methods are statistically indistinguishable from each other. Results from molecular pollen analysis also show that while bees can carry multiple species ofClarkiaat the same time, they often carry one species of pollen. Our work contributes to the growing body of literature aimed at resolving how pollinators use floral resources. We suggest our novel relative amplicon quantification method as another tool in the developing molecular ecology and pollination biology toolbox.