Neurotransplantation of magnetically labeled oligodendrocyte progenitors: Magnetic resonance tracking of cell migration and myelination
Neurotransplantation of magnetically labeled oligodendrocyte progenitors: Magnetic resonance tracking of cell migration and myelination
复制标题
DOI：
10.1073/pnas.96.26.15256
复制
发表时间：
1999-12-21
影响因子：
11.1
通讯作者：
Frank, JA
中科院分区：
文献类型：
--
作者：
Bulte, JWM;Zhang, SC;Frank, JA
脱髓鞘是人类神经系统疾病的常见病理表现，并且经常由于内源性修复失败而持续存在。移植的少突胶质细胞及其前体细胞可以使轴突（重新）髓鞘化，从而提高了治疗干预的可能性。移植细胞的迁移能力对于确定（再）髓鞘形成的程度至关重要，目前只能通过使用侵入性和不可逆的程序来评估。我们利用转铁蛋白受体作为磁性纳米粒子的有效细胞内递送装置，并将标记的少突胶质细胞祖细胞移植到髓磷脂缺陷大鼠的脊髓中。使用三维磁共振显微镜可以轻松检测细胞迁移，对比度增强区域与髓鞘形成程度之间存在密切相关性。目前的结果表明，磁共振追踪移植的少突胶质细胞祖细胞是可行的；该技术有可能轻松扩展到涉及不同前体细胞类型的其他神经移植研究。
Demyelination is a common pathological finding in human neurological diseases and frequently persists as a result of failure of endogenous repair. Transplanted oligodendrocytes and their precursor cells can (re)myelinate axons, raising the possibility of therapeutic intervention. The migratory capacity of transplanted cells is of key importance in determining the extent of (re)myelination and can, at present, be evaluated only by using invasive and irreversible procedures. We have exploited the transferrin receptor as an efficient intracellular delivery device for magnetic nanoparticles, and transplanted tagged oligodendrocyte progenitor cells into the spinal cord of myelin-deficient rats. Cell migration could be easily detected by using three-dimensional magnetic resonance microscopy, with a close correlation between the areas of contrast enhancement and the achieved extent of myelination. The present results demonstrate that magnetic resonance tracking of transplanted oligodendrocyte progenitors is feasible; this technique has the potential to be easily extended to other neurotransplantation studies involving different precursor cell types.