IMPROVEMENT OF CULTURE CONDITIONS FOR HUMAN MEGAKARYOCYTIC AND PLURIPOTENT PROGENITOR CELLS BY LOW OXYGEN-TENSION
IMPROVEMENT OF CULTURE CONDITIONS FOR HUMAN MEGAKARYOCYTIC AND PLURIPOTENT PROGENITOR CELLS BY LOW OXYGEN-TENSION
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DOI：
10.1002/stem.5530050506
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发表时间：
1987-09-01
期刊：
影响因子：
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通讯作者：
MIZOGUCHI, H
中科院分区：
文献类型：
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作者：
KATAHIRA, J;MIZOGUCHI, H
采用半固体甲基纤维素集落形成法研究了低氧分压对骨髓造血祖细胞生长的影响。在低氧压(5%)下培养的粒-巨噬系祖细胞(CFU-gm)、早期(BFU-e)和晚期(CFU-e)红系祖细胞、巨核系祖细胞(CFU-Meg)和多能祖细胞(CFU-Mix)的克隆性生长显著高于常规空气(20%)。硫醇化合物2-巯基乙醇在常规空气中对菌落生长有很强的相加作用，但在低氧分压下几乎没有或几乎没有影响。这些结果表明，低氧压下菌落生长的增强可能是由于氧中间产物的减少所致。
The effect of low oxygen tension on the growth of human hemopoietic progenitor cells in bone marrow was investigated using the semisolid methylcellulose colony assay. The clonal growth of granulocyte-macrophage progenitors (CFU-gm), early (BFU-e) and late (CFU-e) erythroid progenitors, megakaryocyte progenitors (CFU-meg) and pluripotent progenitors (CFU-mix) improved more markedly in incubation at the low oxygen tension (5%) than in conventional air (20%). The thiol compound 2-mercaptoethanol had a strong additive effect on colony growth in conventional air, but little or no effect in the low oxygen tension. These results suggest that enhancement of colony growth in the low oxygen tension may be due to a decrease in the production of oxygen intermediates.