Bioelectric properties of cultured epithelial monolayers from distal lung of 18-day fetal rat.
Bioelectric properties of cultured epithelial monolayers from distal lung of 18-day fetal rat.
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18 天胎鼠远端肺培养的单层上皮细胞的生物电特性。
DOI：
10.1152/ajplung.1992.262.5.l628
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Gatzy,JT
中科院分区：
文献类型：
--
作者：
Barker,PM;Stiles,AD;Boucher,RC;Gatzy,JT
对胎羊的体内研究表明，整个胎儿期肺腔中存在的液体源自肺上皮分泌的 Cl-。来自胎儿远端（妊娠 18 天）肺的富集上皮细胞的单层制备物，在无血清培养基中生长，在组织学上与新鲜组织的腺泡（前肺泡）结构相似。在 Ussing 室中，基础跨上皮电位差 (PD)、计算的等效短路电流 (Ieq) 和跨上皮电阻 (R) 分别为 4.4 +/- 0.3 mV（基质正）、35.6 +/- 1.6 microA/cm2 和 120.0 +/- 4.0 Ω cm2。哇巴因 (10(-3) M) 在 30 分钟内消除了 57% 的基础 Ieq，阿米洛利 (10(-4) M) 导致 Ieq 下降 13%，根皮苷 (10(-4) M) 对生物电特性没有影响。二苯胺-2-羧酸盐（DPC，3 x 10(-3) M）抑制 Ieq 50%。布美他尼对基线生物电参数没有影响。伴随心尖或双侧 Cl- 替代并被特布他林增强的超极化表明心尖 Cl- 渗透选择性。 Na+ 替代对阿米洛利预处理的单层细胞的影响与 Na(+) 依赖性 Cl 分泌或阿米洛利不敏感途径一致。在这些生长条件下，该制剂表现出与 Cl- 分泌和 Na+ 吸收相容的生物电特性。 Cl- 分泌的机制可能与气道相似，但独特的是布美他尼不敏感。
In vivo studies of fetal sheep suggest that the liquid present in the lumen of the lung throughout fetal life is derived from Cl- secretion by the pulmonary epithelium. Monolayer preparations of enriched epithelial cells from distal fetal rat (18-day gestation) lung, grown in serum-free media, were histologically similar to acinar (prealveolar) structures of fresh tissue. In Ussing chambers, basal transepithelial potential difference (PD), calculated equivalent short-circuit current (Ieq), and transepithelial resistance (R) were 4.4 +/- 0.3 mV (matrix positive), 35.6 +/- 1.6 microA/cm2, and 120.0 +/- 4.0 omega cm2, respectively. Ouabain (10(-3) M) eliminated 57% of basal Ieq within 30 min, amiloride (10(-4) M) induced a 13% fall in Ieq, and phlorizin (10(-4) M) had no effect on bioelectric properties. Diphenylamine-2-carboxylate (DPC, 3 x 10(-3) M) inhibited Ieq by 50%. Bumetanide had no effect on baseline bioelectric parameters. The hyperpolarization that accompanied apical or bilateral replacement of Cl- and was enhanced by terbutaline suggested an apical Cl- permselectivity. Effects of Na+ replacement on amiloride-pretreated monolayers were consistent with Na(+)-dependent Cl- secretion or amiloride-insensitive pathways. Under these growth conditions, this preparation exhibits bioelectric characteristics that are compatible with Cl- secretion and Na+ absorption. The mechanism of Cl- secretion may be similar to that of airways but is uniquely bumetanide insensitive.