Outer Membrane Proteins A (OmpA) and X (OmpX) Are Essential for Basolateral Invasion of Cronobacter sakazakii
Outer Membrane Proteins A (OmpA) and X (OmpX) Are Essential for Basolateral Invasion of Cronobacter sakazakii
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DOI：
10.1128/aem.02498-09
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发表时间：
2010-08-01
影响因子：
4.4
通讯作者：
Ryu, Sangryeol
中科院分区：
文献类型：
--
作者：
Kim, Kyumson;Kim, Kwang-Pyo;Ryu, Sangryeol
阪崎克罗诺杆菌是一种主动侵入宿主真核细胞的机会性病原体。为了确定阪崎梭菌肠道易位的侵袭因子，我们首次利用lambda Red重组系统构建了外膜蛋白X （OmpX）和A （OmpA）缺失突变体。ompX和ompA缺失突变体对人肠细胞样上皮Caco-2和人肠上皮INT-407细胞的侵袭明显减少，从大鼠幼仔肝脏和脾脏中回收的突变体细胞明显减少。此外，与完整靶细胞相比，在紧密连接破坏的靶细胞中，突变体的侵袭和初始关联电位以与野生型相似的速度增加，这表明OmpX和OmpA参与了坂崎弧菌的基底外侧侵袭。这是首次报道坂崎梭菌的毒力决定因素，这些决定因素对坂崎梭菌的基底外侧入侵至关重要，可能对其毒力至关重要。
Cronobacter sakazakii is an opportunistic pathogen that actively invades host eukaryotic cells. To identify invasion factors responsible for the intestinal translocation of C. sakazakii, we constructed for the first time outer membrane protein X (OmpX) and A (OmpA) deletion mutants using the lambda Red recombination system. The ompX and ompA deletion mutants showed significantly reduced invasion of human enterocyte-like epithelial Caco-2 and human intestinal epithelial INT-407 cells, and significantly fewer mutant cells were recovered from the livers and spleens of rat pups. Furthermore, compared with intact target cells, the invasion and initial association potentials of the mutants increased at a rate similar to that of the wild type in tight-junction-disrupted target cells, suggesting that OmpX and OmpA are involved in basolateral invasion by C. sakazakii. This is the first report of C. sakazakii virulence determinants that are essential for basolateral invasion and that may be critical for the virulence of C. sakazakii.