Bovine lactoferrin induces both mucosal and systemic immune response in mice
Bovine lactoferrin induces both mucosal and systemic immune response in mice
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DOI：
10.1017/s0022029997002732
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发表时间：
1998-05-01
影响因子：
2.1
通讯作者：
Tomé, D
中科院分区：
文献类型：
--
作者：
Debbabi, H;Dubarry, M;Tomé, D
乳铁蛋白(LF)是一种与铁结合的糖蛋白，在铁的转运中起作用，既是抑菌剂又是生长调节剂。本研究旨在探讨反复口服牛乳乳脂对小鼠免疫应答的影响。每组10只雌性BALB/c小鼠，每天给予两种剂量的蛋白质抗原：低剂量(0.05 mg/g体重/d)或高剂量(1 mg/g体重/d)，或水作为对照。第四组肌肉注射0.01mgLF完全弗氏佐剂。小鼠肠道液和血清中均可检测到抗LFIgA和Ig G抗体。LF组大鼠肠液中总免疫球蛋白高于对照组。血清中未检测到差异。与对照组相比，饲喂LF的小鼠脾和Peyer‘s区的IgA和Ig G分泌增加。在体外，75mUg/mlLF组小鼠脾细胞和Peyer氏斑细胞对[H-3]胸腺嘧啶核苷的摄取均高于对照组，但LF组的增殖速率高于对照组。这些结果表明，LF可作为黏膜免疫系统的免疫刺激因子，粘膜免疫系统的激活依赖于LF与肠粘膜结合的能力。
Lactoferrin (Lf) is a milli iron-binding glycoprotein that plays a role in iron transport and acts as both a bacteriostatic and a growth modulating agent. The aim of this study was to investigate the nature of immune responses induced by repeated oral administration of bovine milk Lf in mice. Groups of ten female BALB/c mice were fed daily for 4 weeks with two doses of protein antigen: a low (0.05 mg/g body weight per d) or high (1 mg/g body weight per d) dose of Lf, or water as a control. A fourth group was immunized intramuscularly with 0.01 mg Lf in complete Freund's adjuvant. Anti-Lf IgA and IgG were detected in the intestinal fluid and serum of mice given Lf. Total immunoglobulins were higher in the intestinal fluid in Lf groups than in the control group. No difference could be detected in the serum. IgA and IgG secretion was enhanced in Peyer's patches and spleen from Lf-fed mice, in comparison with controls. [H-3]thymidine uptake into Peyer's patch and spleen cells from both control and Lf-fed mice was enhanced by 75 mu g Lf/ml in vitro, but Lf groups had a greater proliferation rate than the control group. These findings suggested that Lf could act as an immunostimulating factor on the mucosal immune system and that activation of the mucosal immune system is dependent on the ability of Lf to bind to the intestinal mucosa.