Performance and microbial community analysis of a novel DEAMOX based on partial-denitrification and anammox treating ammonia and nitrate wastewaters
Performance and microbial community analysis of a novel DEAMOX based on partial-denitrification and anammox treating ammonia and nitrate wastewaters
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基于部分反硝化和厌氧氨氧化的新型 DEAMOX 处理氨氮和硝酸盐废水的性能和微生物群落分析
DOI：
10.1016/j.watres.2016.10.051
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发表时间：
2017-01-01
期刊：
影响因子：
12.8
通讯作者：
Peng, Yongzhen
中科院分区：
文献类型：
--
作者：
Du, Rui;Cao, Shenbin;Peng, Yongzhen
在本研究中，开发了一种新型的DEAMOX（反硝化氨氧化）工艺，该工艺将厌氧氨氧化与从硝酸盐（NO₃⁻ -N）产生亚硝酸盐（NO₂⁻ -N）的部分反硝化相耦合，用于同时处理含氨（NH₄⁺ -N）和NO₃⁻ -N的废水。在序批式反应器（SBR）中，采用不同的碳源（乙酸盐（R1）和乙醇（R2））进行部分反硝化来评估其性能。长期运行（180天）表明，两个反应器都实现了理想的脱氮效果。尽管温度季节性下降（29.2℃ - 12.7℃），R1中的性能仍保持稳定，在进水NO₃⁻ -N与NH₄⁺ -N的比例（NO₃⁻ -N/NH₄⁺ -N）为1.0时，平均获得了93.6%的高脱氮效率（NRE）。在R1中，厌氧氨氧化过程对总氮（TN）去除的贡献率超过95%，部分反硝化过程中硝酸盐到亚硝酸盐的转化率（NTR）为95.8%。在R2中观察到NRE略低，随着温度从22.7℃时的90.0%下降到16.6℃时的85.2%，这是由于NTR降低（从87.0% - 67.0%）。高通量测序分析表明，陶厄氏菌属在两个SBR中均占优势（在R1中占61.53%，在R2中占45.17%），并且可能对高NO₂⁻ -N积累的部分反硝化起关键作用。在R2中发现了能够完全反硝化（NO₃⁻ -N → N₂）的反硝化丝菌属，这可能导致较低的NTR。此外，在R1中检测到不同的厌氧氨氧化菌种，有暂定分类单元布罗卡迪亚菌和暂定分类单元库恩菌，而在R2中只有暂定分类单元库恩菌。（C）2016爱思唯尔有限公司。保留所有权利。
In this study, a novel DEAMOX (DEnitrifying AMmonium OXidation) process coupling anammox with partial-denitrification generated nitrite (NO2--N) from nitrate (NO3--N) was developed for simultaneously treating ammonia (NH4+-N) and NO3--N containing wastewaters. The performance was evaluated in sequencing batch reactors (SBRs) with different carbon sources for partial-denitrification: acetate (R1) and ethanol (R2). Long-term operation (180 days) suggested that desirable nitrogen removal was achieved in both reactors. The performance maintained stably in R1 despite the seasonal decrease of temperature (29.2 degrees C-12.7 degrees C), and high nitrogen removal efficiency (NRE) of 93.6% on average was obtained with influent NO3--N to NH4+-N ratio (NO3--N/NH4+-N) of 1.0. The anammox process contributed above 95% to total nitrogen (TN) removal in R1 with the nitrate-to-nitrite transformation ratio (NTR) of 95.8% in partial-denitrification. A little lower NRE was observed in R2 with temperature dropped from 90.0% at 22.7 degrees C to 85.2% at 16.6 degrees C due to the reduced NTR (87.0%-67.0%). High-throughput sequencing analysis revealed that Thauera genera were dominant in both SBRs (accounted for 61.53% in R1 and 45.17% in R2) and possibly played a key role for partial-denitrification with high NO2--N accumulation. The Denitratisoma capable of complete denitrification (NO3--N -> N-2) was found in R2 that might lead to lower NTR. Furthermore, different anammox species was detected with Candidatus Brocadia and Candidatus Kuenerda in R1, and only Candidatus Kuenenia in R2. (C) 2016 Elsevier Ltd. All rights reserved.