REM sleep deprivation in monocularly occluded kittens reduces the size of cells in LGN monocular segment.
REM sleep deprivation in monocularly occluded kittens reduces the size of cells in LGN monocular segment.
复制标题
单眼闭塞小猫的快速眼动睡眠剥夺会减少 LGN 单眼节段的细胞大小。
DOI：
10.1093/sleep/21.8.837
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发表时间：
1998
期刊：
影响因子：
5.6
通讯作者：
Roffwarg,HP
中科院分区：
文献类型：
--
作者：
Shaffery,JP;Oksenberg,A;Marks,GA;Speciale,SG;Mihailoff,G;Roffwarg,HP
研究目的在本研究中，我们验证了这样一个假设：当rem状态激活被剥夺时（无论是否刺激眼睛，rem状态激活都会影响到所有外侧腿状核层），由于外在和内在激活的丧失，与视觉输入隔绝并被剥夺rem状态激活的单眼节段（MS）将表现出更小的细胞。我们进行了一项研究，比较单眼剥夺(MD) +快速眼动剥夺（RD）的MSs小猫与两个年龄匹配的非RD组，MD only组和MD MOMS组（生活在家里笼子里的MD小猫）的体细胞大小。测量结果双侧MSs各100个细胞的核外轮廓。正如预测的那样，MD+RD小猫贴膜眼的MS的平均细胞大小明显小于未贴膜眼的MS，但在对照组中没有。单因素方差分析比较各组同侧MS细胞大小均值也有显著性，但两种MS在事后检验中显示不同的结果。MS细胞大小的顺序与一项测量显著相关，该测量聚集了达到特定MS的激活源及其持续时间。这些结果表明，在中枢神经系统发育过程中，移除快速眼动状态激活会放大正常视觉传入中断时产生的可塑性过程。我们在LGN的MS中发现，在快速眼动睡眠的通常操作中，中央视觉感觉部位接受内在激活，在视觉系统中，这是外部来源刺激的附加和补充。在早期发育过程中，快速眼动睡眠期间中央视觉区域的规范对称激活可以抵消感官刺激缺失或异常引起的可塑性变化。
Study ObjectivesIn this study, we test the hypothesis that when REM-state activation (which impinges upon all lateral geniculate nucleus laminae irrespective of stimulating eye) is deprived, the monocular segment (MS) that is cut off from visual input and also deprived of REM-state activation will exhibit smaller cells, owing to the loss of extrinsic as well as intrinsic activation.DesignWe carried out a study comparing soma sizes in the MSs of kittens subjected to monocular deprivation (MD) + REM deprivation (RD) to two age-matched nonRD groups, MD ONLYs and MD MOMS (MD kittens living in their home cages).Measurements and ResultsPerikaryal outlines of 100 cells in each of the bilateral MSs were measured. As predicted, mean cell size in the MS connected to the patched eye of MD+RD kittens, but in neither of the control groups, was significantly smaller than in the MS afferented by the nonpatched eye. One-way ANOVAs comparing MS cell-size means from the same sides across groups were also significant, but the two MSs showed different results on post hoc tests. The ordering of MS cell-size means correlated significantly with a measure that aggregates the sources of activation reaching a particular MS and their durations.ConclusionsThese results reveal that removal of REM-state activation during CNS development amplifies the plasticity processes generated when normal visual afferentation to central visual areas is interrupted. Our findings in the MS of the LGN indicate that during the usual operation of REM sleep, central visual-sensory sites receive intrinsic activation that, in the visual system, is additive and complementary to the stimulation obtained from extrinsic sources. In the course of early development, normative symmetrical activation of central visual areas during REM sleep may counterbalance plasticity changes caused either by absent or aberrant sensory stimulation.